Team:Exeter/lab book/3gip/wk6

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Latest revision as of 00:06, 27 September 2012

ExiGEM2012 Lab Book 3GP wk6

The 3-Gene Inducible Plasmid: 13th - 17th August 2012

Monday 13th August

See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005)


Tuesday 14th August

See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005)


Wednesday 15th August

Morning

•Miniprepping

BBa_J13002 + wbnK BBa_B0014 in pSB1T3

wfcA + BBa_B0014 in pSB1T3

wbnJ + BBa_B0014 in pSB1T3

BBa_B0034 wbbC + BBa_B0014 in pSB1T3

Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed.

After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation.

40ul of water was added instead of 50ul in order to make sure the concentration of DNA increased.

All were nanodropped and only wfcA + BBa_B0014 and wbnJ + BBa_B0014 gave good concentrations.


Wednesday 15th August – Friday 17th August

Preparation for presentation so no lab work.

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