Team:ZJU-China/project.htm

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<img src="https://static.igem.org/mediawiki/igem.org/a/ad/Zju_library_Fig2a.jpg" width="500px" />
<img src="https://static.igem.org/mediawiki/igem.org/a/ad/Zju_library_Fig2a.jpg" width="500px" />
<p>fig2a. MS2 and PP7 bind to the scaffold and make GFP work. </p>
<p>fig2a. MS2 and PP7 bind to the scaffold and make GFP work. </p>
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<p>&nbsp;</p>
<img src="https://static.igem.org/mediawiki/igem.org/9/98/Zju_library_Fig2b.jpg" width="500px" />
<img src="https://static.igem.org/mediawiki/igem.org/9/98/Zju_library_Fig2b.jpg" width="500px" />
<p>fig2b. By mutating aptamer binding site, scaffolding is stop. </p>
<p>fig2b. By mutating aptamer binding site, scaffolding is stop. </p>
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<p>&nbsp;</p>
<img src="https://static.igem.org/mediawiki/igem.org/3/33/Zju_library_fig2c.jpg" width="500px" />
<img src="https://static.igem.org/mediawiki/igem.org/3/33/Zju_library_fig2c.jpg" width="500px" />
<p>fig2c. significant difference between D0 an D0M3</p>
<p>fig2c. significant difference between D0 an D0M3</p>
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<p>2.2 Protein expression (mRNA) regulation: RNA scaffold as a free molecular in cell can specific bind mRNA and protein. Binding molecular changes the structure of scaffold to release or combine something. So that oncogene and virogene can be found and controlled by the drug from RNA scaffold. The problem of cancer therapeutic drug side effecting may solved by it. </p>
<p>2.2 Protein expression (mRNA) regulation: RNA scaffold as a free molecular in cell can specific bind mRNA and protein. Binding molecular changes the structure of scaffold to release or combine something. So that oncogene and virogene can be found and controlled by the drug from RNA scaffold. The problem of cancer therapeutic drug side effecting may solved by it. </p>
<p>&nbsp;</p>
<p>&nbsp;</p>
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<p>2.3 Self quenching(Self regulation): Adding self binding site, a balance of “on” and “off” scaffolds is built. The relationship between the binding site size, CG bases, binding form and the rate binding molecular is urgently modeled. Forming dimerization and trimerization, the concentration of working scaffold could be regulated.</p> \
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<p>2.3 Self quenching(Self regulation): Adding self binding site, a balance of “on” and “off” scaffolds is built. The relationship between the binding site size, CG bases, binding form and the rate binding molecular is urgently modeled. Forming dimerization and trimerization, the concentration of working scaffold could be regulated.</p>  
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>2.4 Polo-scaffold: Scaffold with intermolecular binding component. These scaffolds bind each other or bind through mediate molecular. And this binding mode has been proved both in vitro and vivo. The aggregation of molecular also makes artificial organelle achievable. </p>
<p>2.4 Polo-scaffold: Scaffold with intermolecular binding component. These scaffolds bind each other or bind through mediate molecular. And this binding mode has been proved both in vitro and vivo. The aggregation of molecular also makes artificial organelle achievable. </p>

Revision as of 23:54, 26 September 2012

PROJECT

01 ABSTRACT

02 BACKGROUND

03 S0: BASIC RNA SCAFFOLD

04 S1: RIBOSCAFFOLD

05 S2: SCAFFOLD LIBRARY

06 S3: BIOSYNTHESIS OF IAA

07 PARTS

08 RESULTS

09 PERSPECTIVES