"
Team:UT-Tokyo/Project/H2 E.coli/Discussion
From 2012.igem.org
Minakuchi.T (Talk | contribs) (→The Efffects of the E363G Mutation in fhlA Gene on Hydrogen Production) |
|||
| Line 4: | Line 4: | ||
</p><img id="abstimg" src="https://static.igem.org/mediawiki/2012/d/d3/UT-Tokyo_blank01.png" alt="box-background image" /><p> | </p><img id="abstimg" src="https://static.igem.org/mediawiki/2012/d/d3/UT-Tokyo_blank01.png" alt="box-background image" /><p> | ||
<!-- ここから</p>の前までを編集してください --> | <!-- ここから</p>の前までを編集してください --> | ||
| - | + | Here we analyze the results and describe future applications. | |
</p></div><p></html> | </p></div><p></html> | ||
<!-- 以下テンプレ部分まで自由記述 --> | <!-- 以下テンプレ部分まで自由記述 --> | ||
== The Effects of the E363G Mutation in fhlA Gene on Hydrogen Production == | == The Effects of the E363G Mutation in fhlA Gene on Hydrogen Production == | ||
| - | Sanchez-Torres et al. obtained by random mutagenesis several mutants of the transcriptional factor gene fhlA in which hydrogen gas production is enhanced.[1] Among these mutants, the E363G mutation allowed ''E.coli'' cells to produce hydrogen six times more than the original fhlA under anaerobic conditions with 20 mM formate. Therefore, we made a BioBrick part named fhlA-E363G by introducing the same mutation into the original wild type fhlA gene which we BioBricked. | + | Sanchez-Torres et al. obtained by random mutagenesis several mutants of the transcriptional factor gene ''fhlA'' in which hydrogen gas production is enhanced.[[#References|[1]]] Among these mutants, the E363G mutation allowed ''E.coli'' cells to produce hydrogen six times more than the original ''fhlA'' under anaerobic conditions with 20 mM formate. Therefore, we made a BioBrick part named ''fhlA'' -E363G by introducing the same mutation into the original wild type ''fhlA'' gene which we BioBricked. |
| - | Our aim is to make ''E. coli'' cells which are capable of producing hydrogen in a glucose-rich environment.Hydrogen production was elevated in the fhlA overexpression cells compared to the wild type. In an attempt to further increase hydrogen production, we compared the hydrogen production of two ''E. coli'' strains which over-expresses wild-type fhlA and fhlA-E363G in a glucose-rich environment. As a result, the fhlA-E363G expressing strain generated less hydrogen gas than the wild type fhlA expressing strain. It is considered that under glucose-rich conditions the E363G mutation in fhlA does not lead to an increase of hydrogen production compared to wild type fhlA. | + | Our aim is to make ''E. coli'' cells which are capable of producing hydrogen in a glucose-rich environment.Hydrogen production was elevated in the ''fhlA'' overexpression cells compared to the wild type. In an attempt to further increase hydrogen production, we compared the hydrogen production of two ''E. coli'' strains which over-expresses wild-type ''fhlA'' and ''fhlA'' -E363G in a glucose-rich environment. As a result, the ''fhlA'' -E363G expressing strain generated less hydrogen gas than the wild type ''fhlA'' expressing strain. It is considered that under glucose-rich conditions the E363G mutation in ''fhlA'' does not lead to an increase of hydrogen production compared to wild type ''fhlA''. |
| - | |||
== The Effects of Formate on Hydrogen Production == | == The Effects of Formate on Hydrogen Production == | ||
We obtained the results which indicate that formate do not have significant effect on hydrogen production. The results do not support previous study, which is not conflicting to the project. | We obtained the results which indicate that formate do not have significant effect on hydrogen production. The results do not support previous study, which is not conflicting to the project. | ||
| - | The results show that fhlA overexpression elevate hydrogen production, but there was no correlation between production efficiency and formate addition or medium and E363G mutation, which is unexpected. In order to make system more efficient, further investigation on these factors is needed. | + | The results show that ''fhlA'' overexpression elevate hydrogen production, but there was no correlation between production efficiency and formate addition or medium and E363G mutation, which is unexpected. In order to make system more efficient, further investigation on these factors is needed. |
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | == | + | === References === |
| - | + | [1]Viviana Sanchez-Torres, Toshinari Maeda, and Thomas K. Wood. Protein Engineering of the Transcriptional Activator FhlA To Enhance Hydrogen Production in Escherichia coli. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75, 5639–5646. 2009. | |
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<!-- 以上自由記述 --> | <!-- 以上自由記述 --> | ||
| Line 60: | Line 34: | ||
{{:Team:UT-Tokyo/Template/Side|twdisp=block}} | {{:Team:UT-Tokyo/Template/Side|twdisp=block}} | ||
<!-- 以下サイドメニュー編集 ;の後には、もしこのページが第2階層にあれば、そのページ内の見出しを羅列。第1階層にあれば、そのカテゴリ内の第2階層ページ名を羅列する。;の後には名称を、:の後にはその説明を記述する。:と;のセットは適当に増減してください --> | <!-- 以下サイドメニュー編集 ;の後には、もしこのページが第2階層にあれば、そのページ内の見出しを羅列。第1階層にあれば、そのカテゴリ内の第2階層ページ名を羅列する。;の後には名称を、:の後にはその説明を記述する。:と;のセットは適当に増減してください --> | ||
| - | ;[[# | + | ;[[#The Effects of the E363G Mutation in fhlA Gene on Hydrogen Production|The Effects of the E363G Mutation in fhlA Gene on Hydrogen Production]] |
| - | : | + | : |
| - | ;[[# | + | ;[[#The Effects of Formate on Hydrogen Production|The Effects of Formate on Hydrogen Production]] |
| - | : | + | : |
| - | ; | + | ;[[#References|References]] |
| - | : | + | : |
| - | + | ||
| - | + | ||
<!-- ここまでサイドメニュー編集を --> | <!-- ここまでサイドメニュー編集を --> | ||
<html></p> | <html></p> | ||
Revision as of 23:17, 26 September 2012
H2 E.coli: Discussion
Here we analyze the results and describe future applications.
The Effects of the E363G Mutation in fhlA Gene on Hydrogen Production
Sanchez-Torres et al. obtained by random mutagenesis several mutants of the transcriptional factor gene fhlA in which hydrogen gas production is enhanced.[1] Among these mutants, the E363G mutation allowed E.coli cells to produce hydrogen six times more than the original fhlA under anaerobic conditions with 20 mM formate. Therefore, we made a BioBrick part named fhlA -E363G by introducing the same mutation into the original wild type fhlA gene which we BioBricked.
Our aim is to make E. coli cells which are capable of producing hydrogen in a glucose-rich environment.Hydrogen production was elevated in the fhlA overexpression cells compared to the wild type. In an attempt to further increase hydrogen production, we compared the hydrogen production of two E. coli strains which over-expresses wild-type fhlA and fhlA -E363G in a glucose-rich environment. As a result, the fhlA -E363G expressing strain generated less hydrogen gas than the wild type fhlA expressing strain. It is considered that under glucose-rich conditions the E363G mutation in fhlA does not lead to an increase of hydrogen production compared to wild type fhlA.
The Effects of Formate on Hydrogen Production
We obtained the results which indicate that formate do not have significant effect on hydrogen production. The results do not support previous study, which is not conflicting to the project.
The results show that fhlA overexpression elevate hydrogen production, but there was no correlation between production efficiency and formate addition or medium and E363G mutation, which is unexpected. In order to make system more efficient, further investigation on these factors is needed.
References
[1]Viviana Sanchez-Torres, Toshinari Maeda, and Thomas K. Wood. Protein Engineering of the Transcriptional Activator FhlA To Enhance Hydrogen Production in Escherichia coli. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75, 5639–5646. 2009.
