Team:Marburg SYNMIKRO/Notebook
From 2012.igem.org
(Difference between revisions)
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: primer: [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]] | : primer: [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]] | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel35.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, 2-3: Enhancer, 4-6: P-RBS, 7-8: GixR, 9-10: Enhancer, (PCR 28.08.2012)]] | : [[File:Syn_MR_Gel35.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, 2-3: Enhancer, 4-6: P-RBS, 7-8: GixR, 9-10: Enhancer, (PCR 28.08.2012)]] | ||
Line 1,037: | Line 1,037: | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel36.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, 2-3: Enhancer, 4-6: P-RBS, 7-8: GixR, 9: Enhancer control, 10: P-RBS control, 11: GixR control, (PCR 29.08.2012)]] | : [[File:Syn_MR_Gel36.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, 2-3: Enhancer, 4-6: P-RBS, 7-8: GixR, 9: Enhancer control, 10: P-RBS control, 11: GixR control, (PCR 29.08.2012)]] | ||
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- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel37.jpg|thumb|800px|center|M:1kb gene ruler plus, 1,3,5:SacB, 4,5: none, (PCR 31.08.2012)]] | : [[File:Syn_MR_Gel37.jpg|thumb|800px|center|M:1kb gene ruler plus, 1,3,5:SacB, 4,5: none, (PCR 31.08.2012)]] | ||
Line 1,168: | Line 1,168: | ||
: pC3.mRFP (24) | : pC3.mRFP (24) | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel38.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-6:CFP-Terminator, 7:CFP control, 8-13:mRFP-Terminator 14: mRFP control, 15-16: none, (colony-PCR 03.09.2012)]] | : [[File:Syn_MR_Gel38.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-6:CFP-Terminator, 7:CFP control, 8-13:mRFP-Terminator 14: mRFP control, 15-16: none, (colony-PCR 03.09.2012)]] | ||
Line 1,241: | Line 1,241: | ||
: P-RBS (6x) | : P-RBS (6x) | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel46.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Scar, 2-6:RBS-Gin, 7:Gin control 8-12: gixR, 13: control, (colony-PCR 12.09.2012)]] | : [[File:Syn_MR_Gel46.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Scar, 2-6:RBS-Gin, 7:Gin control 8-12: gixR, 13: control, (colony-PCR 12.09.2012)]] | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel47.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Scar, 2-6:RBS BBa_B0030, 7-12:P-RBS, 13: control, (colony-PCR 12.09.2012)]] | : [[File:Syn_MR_Gel47.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Scar, 2-6:RBS BBa_B0030, 7-12:P-RBS, 13: control, (colony-PCR 12.09.2012)]] | ||
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: terminator A2 | : terminator A2 | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel48.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-3:GroES-GFP, 4:GroES-GFP control, 5:GroES-CFP, 6:GroES-CFP control, 7-10: GroES-Terminator, 11: GroES-Terminator control, 12-15: Enhancer, 16: Enhancer control, (control digest 12.09.2012)]] | : [[File:Syn_MR_Gel48.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-3:GroES-GFP, 4:GroES-GFP control, 5:GroES-CFP, 6:GroES-CFP control, 7-10: GroES-Terminator, 11: GroES-Terminator control, 12-15: Enhancer, 16: Enhancer control, (control digest 12.09.2012)]] | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel49.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:AmiC-mRFP, 3:AmiC-mRFP control, 4-5:AmiC-GFP, 6:AmiC-GFP control, 7-11: GFP-Terminator, 12: GFP-Terminator control, 13-15: Terminator, 16: Terminator control, (control digest 12.09.2012)]] | : [[File:Syn_MR_Gel49.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:AmiC-mRFP, 3:AmiC-mRFP control, 4-5:AmiC-GFP, 6:AmiC-GFP control, 7-11: GFP-Terminator, 12: GFP-Terminator control, 13-15: Terminator, 16: Terminator control, (control digest 12.09.2012)]] | ||
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: primer [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]] | : primer [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]] | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel50.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:RBS-Gin HF, 2:RBS-Gin GC, 3:RBS-Gin SM (PCR 13.09.2012)]] | : [[File:Syn_MR_Gel50.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:RBS-Gin HF, 2:RBS-Gin GC, 3:RBS-Gin SM (PCR 13.09.2012)]] | ||
Line 1,322: | Line 1,322: | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel51.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-4:AmiC-Terminator, 5-6:GroES-Terminator, 7:Scar, 8: control, (colony-PCR 13.09.2012)]] | : [[File:Syn_MR_Gel51.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-4:AmiC-Terminator, 5-6:GroES-Terminator, 7:Scar, 8: control, (colony-PCR 13.09.2012)]] | ||
Line 1,348: | Line 1,348: | ||
: RBS (BBa_B0030) [clone 1 and 3] | : RBS (BBa_B0030) [clone 1 and 3] | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel52.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:AmiC, 2:GroES, 3:none, 4:GFP, 5:CFP, 6:mRFP,(digest 13.-14.09.2012)]] | : [[File:Syn_MR_Gel52.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:AmiC, 2:GroES, 3:none, 4:GFP, 5:CFP, 6:mRFP,(digest 13.-14.09.2012)]] | ||
Line 1,355: | Line 1,355: | ||
: mRFP | : mRFP | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel53.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, (digest 14.09.2012)]] | : [[File:Syn_MR_Gel53.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, (digest 14.09.2012)]] | ||
Line 1,391: | Line 1,391: | ||
: SacB | : SacB | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel54.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:AmiC control, 2:AmiC, 3:GroES control, 4:GroES, 5:CFP control, 6:CFP, 7:SacB control, 8:SacB (digest 17.09.2012)]] | : [[File:Syn_MR_Gel54.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:AmiC control, 2:AmiC, 3:GroES control, 4:GroES, 5:CFP control, 6:CFP, 7:SacB control, 8:SacB (digest 17.09.2012)]] | ||
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: P108-RBS pSB1C3 --> 200,5 ng/µl, 177,8 ng/µl | : P108-RBS pSB1C3 --> 200,5 ng/µl, 177,8 ng/µl | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel55.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:GixR, 3-4:RBS BBa_B0030, 3-4:P108-RBS, (mini prep 17.09.2012)]] | : [[File:Syn_MR_Gel55.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:GixR, 3-4:RBS BBa_B0030, 3-4:P108-RBS, (mini prep 17.09.2012)]] | ||
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: primer [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]] | : primer [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]] | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel57.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:RBS-Gin HF, 3:RBS-Gin GC, 4:RBS-Gin SM, 5: Scar, 7-10:2xTerminator, 11:control, (colony-pcr 18.09.2012)]] | : [[File:Syn_MR_Gel57.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:RBS-Gin HF, 3:RBS-Gin GC, 4:RBS-Gin SM, 5: Scar, 7-10:2xTerminator, 11:control, (colony-pcr 18.09.2012)]] | ||
Line 1,523: | Line 1,523: | ||
: RBS-Gin | : RBS-Gin | ||
- | ; 1% Agarose | + | ; 1% Agarose gel |
: [[File:Syn_MR_Gel58.jpg|thumb|800px|center|M:1kb gene ruler plus, 1,3,5,7:2xTerminator, 2,4,6,8:2xTerminator control, 9,11,13,15:RBS-Gin, 10,12,14,16:RBS-Gin control, (control digest 20.09.2012)]] | : [[File:Syn_MR_Gel58.jpg|thumb|800px|center|M:1kb gene ruler plus, 1,3,5,7:2xTerminator, 2,4,6,8:2xTerminator control, 9,11,13,15:RBS-Gin, 10,12,14,16:RBS-Gin control, (control digest 20.09.2012)]] | ||
Revision as of 23:03, 26 September 2012
Contents[hide] |
Week 1
[all]
09.07. - 15.07.2012
Week 2
16.07. - 22.07.2012
Week 3
23.07. - 29.07.2012
Week 4
30.07. - 05.08.2012
Week 5
06.08. - 12.08.2012
Week 6
13.08. - 19.08.2012
Week 7
20.08. - 26.08.2012
Week 8
27.08. - 02.09.2012
Week 9
03.09. - 09.09.2012
Week 10
10.09. - 16.09.2012
Week 11
17.09. - 23.09.2012