Team:Marburg SYNMIKRO/Notebook

From 2012.igem.org

(Difference between revisions)
Line 1,011: Line 1,011:
: primer: [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]]
: primer: [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]]
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel35.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, 2-3: Enhancer, 4-6: P-RBS, 7-8: GixR, 9-10: Enhancer, (PCR 28.08.2012)]]
: [[File:Syn_MR_Gel35.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, 2-3: Enhancer, 4-6: P-RBS, 7-8: GixR, 9-10: Enhancer, (PCR 28.08.2012)]]
Line 1,037: Line 1,037:
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel36.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, 2-3: Enhancer, 4-6: P-RBS, 7-8: GixR, 9: Enhancer control, 10: P-RBS control, 11: GixR control, (PCR 29.08.2012)]]
: [[File:Syn_MR_Gel36.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, 2-3: Enhancer, 4-6: P-RBS, 7-8: GixR, 9: Enhancer control, 10: P-RBS control, 11: GixR control, (PCR 29.08.2012)]]
Line 1,095: Line 1,095:
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel37.jpg|thumb|800px|center|M:1kb gene ruler plus, 1,3,5:SacB, 4,5: none, (PCR 31.08.2012)]]
: [[File:Syn_MR_Gel37.jpg|thumb|800px|center|M:1kb gene ruler plus, 1,3,5:SacB, 4,5: none, (PCR 31.08.2012)]]
Line 1,168: Line 1,168:
: pC3.mRFP (24)
: pC3.mRFP (24)
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel38.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-6:CFP-Terminator, 7:CFP control, 8-13:mRFP-Terminator 14: mRFP control, 15-16: none, (colony-PCR 03.09.2012)]]
: [[File:Syn_MR_Gel38.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-6:CFP-Terminator, 7:CFP control, 8-13:mRFP-Terminator 14: mRFP control, 15-16: none, (colony-PCR 03.09.2012)]]
Line 1,241: Line 1,241:
: P-RBS (6x)
: P-RBS (6x)
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel46.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Scar, 2-6:RBS-Gin, 7:Gin control 8-12: gixR, 13: control, (colony-PCR 12.09.2012)]]
: [[File:Syn_MR_Gel46.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Scar, 2-6:RBS-Gin, 7:Gin control 8-12: gixR, 13: control, (colony-PCR 12.09.2012)]]
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel47.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Scar, 2-6:RBS BBa_B0030, 7-12:P-RBS, 13: control, (colony-PCR 12.09.2012)]]
: [[File:Syn_MR_Gel47.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Scar, 2-6:RBS BBa_B0030, 7-12:P-RBS, 13: control, (colony-PCR 12.09.2012)]]
Line 1,259: Line 1,259:
: terminator A2
: terminator A2
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel48.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-3:GroES-GFP, 4:GroES-GFP control, 5:GroES-CFP, 6:GroES-CFP control, 7-10: GroES-Terminator, 11: GroES-Terminator control, 12-15: Enhancer, 16: Enhancer control, (control digest 12.09.2012)]]
: [[File:Syn_MR_Gel48.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-3:GroES-GFP, 4:GroES-GFP control, 5:GroES-CFP, 6:GroES-CFP control, 7-10: GroES-Terminator, 11: GroES-Terminator control, 12-15: Enhancer, 16: Enhancer control, (control digest 12.09.2012)]]
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel49.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:AmiC-mRFP, 3:AmiC-mRFP control, 4-5:AmiC-GFP, 6:AmiC-GFP control, 7-11: GFP-Terminator, 12: GFP-Terminator control, 13-15: Terminator, 16: Terminator control, (control digest 12.09.2012)]]
: [[File:Syn_MR_Gel49.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:AmiC-mRFP, 3:AmiC-mRFP control, 4-5:AmiC-GFP, 6:AmiC-GFP control, 7-11: GFP-Terminator, 12: GFP-Terminator control, 13-15: Terminator, 16: Terminator control, (control digest 12.09.2012)]]
Line 1,296: Line 1,296:
: primer [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]]
: primer [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]]
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel50.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:RBS-Gin HF, 2:RBS-Gin GC, 3:RBS-Gin SM (PCR 13.09.2012)]]
: [[File:Syn_MR_Gel50.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:RBS-Gin HF, 2:RBS-Gin GC, 3:RBS-Gin SM (PCR 13.09.2012)]]
Line 1,322: Line 1,322:
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel51.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-4:AmiC-Terminator, 5-6:GroES-Terminator, 7:Scar, 8: control, (colony-PCR 13.09.2012)]]
: [[File:Syn_MR_Gel51.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-4:AmiC-Terminator, 5-6:GroES-Terminator, 7:Scar, 8: control, (colony-PCR 13.09.2012)]]
Line 1,348: Line 1,348:
: RBS (BBa_B0030) [clone 1 and 3]
: RBS (BBa_B0030) [clone 1 and 3]
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel52.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:AmiC, 2:GroES, 3:none, 4:GFP, 5:CFP, 6:mRFP,(digest 13.-14.09.2012)]]
: [[File:Syn_MR_Gel52.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:AmiC, 2:GroES, 3:none, 4:GFP, 5:CFP, 6:mRFP,(digest 13.-14.09.2012)]]
Line 1,355: Line 1,355:
: mRFP
: mRFP
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel53.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, (digest 14.09.2012)]]
: [[File:Syn_MR_Gel53.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:Terminator, (digest 14.09.2012)]]
Line 1,391: Line 1,391:
: SacB
: SacB
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel54.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:AmiC control, 2:AmiC, 3:GroES control, 4:GroES, 5:CFP control, 6:CFP, 7:SacB control, 8:SacB (digest 17.09.2012)]]
: [[File:Syn_MR_Gel54.jpg|thumb|800px|center|M:1kb gene ruler plus, 1:AmiC control, 2:AmiC, 3:GroES control, 4:GroES, 5:CFP control, 6:CFP, 7:SacB control, 8:SacB (digest 17.09.2012)]]
Line 1,415: Line 1,415:
: P108-RBS pSB1C3 --> 200,5 ng/µl, 177,8 ng/µl
: P108-RBS pSB1C3 --> 200,5 ng/µl, 177,8 ng/µl
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel55.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:GixR, 3-4:RBS BBa_B0030, 3-4:P108-RBS, (mini prep 17.09.2012)]]
: [[File:Syn_MR_Gel55.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:GixR, 3-4:RBS BBa_B0030, 3-4:P108-RBS, (mini prep 17.09.2012)]]
Line 1,439: Line 1,439:
: primer [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]]
: primer [[Team:Marburg_SYNMIKRO/Primer#MS20|MS20]] + [[Team:Marburg_SYNMIKRO/Primer#MS21|MS21]]
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel57.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:RBS-Gin HF, 3:RBS-Gin GC, 4:RBS-Gin SM, 5: Scar, 7-10:2xTerminator, 11:control, (colony-pcr 18.09.2012)]]
: [[File:Syn_MR_Gel57.jpg|thumb|800px|center|M:1kb gene ruler plus, 1-2:RBS-Gin HF, 3:RBS-Gin GC, 4:RBS-Gin SM, 5: Scar, 7-10:2xTerminator, 11:control, (colony-pcr 18.09.2012)]]
Line 1,523: Line 1,523:
: RBS-Gin
: RBS-Gin
-
; 1% Agarose gele
+
; 1% Agarose gel
: [[File:Syn_MR_Gel58.jpg|thumb|800px|center|M:1kb gene ruler plus, 1,3,5,7:2xTerminator, 2,4,6,8:2xTerminator control, 9,11,13,15:RBS-Gin, 10,12,14,16:RBS-Gin control, (control digest 20.09.2012)]]
: [[File:Syn_MR_Gel58.jpg|thumb|800px|center|M:1kb gene ruler plus, 1,3,5,7:2xTerminator, 2,4,6,8:2xTerminator control, 9,11,13,15:RBS-Gin, 10,12,14,16:RBS-Gin control, (control digest 20.09.2012)]]

Revision as of 23:03, 26 September 2012

Main Page

Contents

[hide]

Week 1

[all]
09.07. - 15.07.2012

Week 2

16.07. - 22.07.2012

Week 3

23.07. - 29.07.2012

Week 4

30.07. - 05.08.2012

Week 5

06.08. - 12.08.2012

Week 6

13.08. - 19.08.2012

Week 7

20.08. - 26.08.2012

Week 8

27.08. - 02.09.2012

Week 9

03.09. - 09.09.2012

Week 10

10.09. - 16.09.2012

Week 11

17.09. - 23.09.2012