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| | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> | | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> |
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| - | Agarose concentration depends on the size of the DNA to be run. | + | Agarose concentration depends on the size of the DNA to be run. We will mostly use 1%. |
| - | Example using 1% agarose gel and small gel box (80 ml of gel):
| + | VOL is the desired volume of gel in ml: |
| | | | |
| - | # Add 0.8 g of agarose to a 80 ml clean glass bottle. | + | # Add 0.01*VOL g of agarose to a clean glass bottle. |
| - | # Pour 1.6 ml of 50xTAE in a graduated cylinder. Fill up to 80 ml with di water. | + | # Pour VOL/50 ml of 50xTAE in a graduated cylinder. Fill up to VOL ml with di water. |
| - | # Add the resulting 80 ml of 1xTAE to the glass bottle with agarose. | + | # Add the resulting VOL ml of 1xTAE to the glass bottle with agarose. |
| | # Microwave, at 7, the bottle (no cap!) until it boils. | | # Microwave, at 7, the bottle (no cap!) until it boils. |
| | # Carefully remove bottle (can be super heated!) and check for the total absence of particles. Microwave again if needed. | | # Carefully remove bottle (can be super heated!) and check for the total absence of particles. Microwave again if needed. |
| - | # Prepare a small gel box and fill it up with the agarose solution (maybe not the whole solutio is needed). | + | # Prepare a gel box and fill it up with the agarose solution (maybe not the whole solution is needed). |
| - | # Add 3 µl (0.05 µl per ml of gel in the box) of Red Gel (it's in the iGEM drawer) and stirr until disolved. | + | # Add 0.05 µl per ml of gel in the box of Red Gel (it's in the iGEM drawer) and stirr until disolved. |
| | # UNFINISHED | | # UNFINISHED |
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| | {{:Team:EPF-Lausanne/Template/ProtocolFooter}} | | {{:Team:EPF-Lausanne/Template/ProtocolFooter}} |
| | <noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | | <noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> |
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