Team:Exeter/Results/GlycoBase

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<p>By searching different databases online we were able to catalogue over 120 glycosyltransferases from different strains of <i>E. coli</i>. We set up the database so that the enzymes could be categorised by their acceptor and donor sugars. From that we were able to determine which sugars we would be able to stick together with the particular glycosyltransferases that we found. We called this database GlycoBase and this was the foundation of our project.</p>
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Revision as of 22:06, 26 September 2012


Glycobase

By searching different databases online we were able to catalogue over 120 glycosyltransferases from different strains of E. coli. We set up the database so that the enzymes could be categorised by their acceptor and donor sugars. From that we were able to determine which sugars we would be able to stick together with the particular glycosyltransferases that we found. We called this database GlycoBase and this was the foundation of our project.

Donor
Acceptor
Bonding
Organism
Branching
Characterised
D-GalNAc Und-P Und-P-P-GlcNAc Escherichia coli
N
NO
D-GalNAc Und-P Und-P-P-GlcNAc Escherichia coli
N
NO
D-GalNAc Und-P Und-P-P-GlcNAc Escherichia coli
N
NO
Alpha-D-Gal Beta-D-GalNAc Alpha-D-Gal(1->3)-Beta-D-GalNAc Escherichia coli
N
YES
Alpha-D-Gal Alpha-L-Fuc Alpha-D-Gal-(1->3)-(Alpha-L-Fuc-(1->2))-Beta-D-Gal Escherichia coli
N
NO
Alpha-D-Gal Alpha-L-FucNAm Alpha-D-Gal-(1->3)-Alpha-L-FucNAm Escherichia coli
N
NO
Beta-D-Gal Beta-D-GalNAc Beta-D-Gal(1->3)-Beta-D-GalNAc Escherichia coli
N
NO
Beta-D-Gal Alpha-D-GalNAc Beta-D-Gal-(1->3)-Alpha-D-GalNAc Escherichia coli
N
NO
Beta-D-Gal Beta-D-Gal Beta-D-Gal-(1->4)-Beta-D-Gal Escherichia coli
Y
NO
Beta-D-Gal Beta-D-Glc Beta-D-Gal-(1->4)-Beta-D-Glc Escherichia coli
N
NO
Beta-D-Gal Beta-D-Galf Beta-D-Gal-(1->6)-Beta-D-Galf Escherichia coli
N
NO
Alpha-D-GalA Alpha-L-Fuc Alpha-D-GalA-(1->3)-Alpha-L-Fuc Shigella dysenteriae
N
NO
Beta-D-GalA Alpha-D-GlcNAc Beta-D-GalA-(1->3)-Alpha-D-GlcNAc Shigella boydii
N
NO
Alpha-D-GalNAc Alpha-D-GalNAc Alpha-D-GalNAc-(1->3)-Alpha-D-GalNAc Escherichia coli
N
NO
Beta-D-GalNAc Alpha-D-Gal Beta-D-GalNAc(1->4)-Alpha-D-Gal Escherichia coli
N
YES
Alpha-L-Fuc Beta-D-Gal Alpha-L-Fuc-(1->2)-Beta-D-Gal Escherichia coli
Y
YES
Alpha-L-FucNAc Alpha-D-Glc Alpha-L-FucNAc-(1->4)-Alpha-D-Glc Escherichia coli
N
NO
Alpha-L-FucNAc Alpha-D-GlcNAc Alpha-L-FucNAc-(1->3)-Alpha-D-GlcNAc Escherichia coli
N
NO
Alpha-L-FucNAm Beta-D-GlcNAc Alpha-L-FucNAm-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Alpha-L-Rha Alpha-L-Rha Alpha-L-Rha-(1->3)-Alpha-L-Rha Escherichia coli
N
NO
Alpha-L-Rha Alpha-L-Rha Alpha-L-Rha-(1->2)-Alpha-L-Rha  Escherichia coli
N
NO
Alpha-L-Rha Alpha-D-Glc Alpha-L-Rha-(1->6)-Alpha-D-Glc Escherichia coli
N
NO
Alpha-L-Rha Alpha-D-Man Alpha-L-Rha-(1->3)-Alpha-D-Man Escherichia coli
Y
NO
Alpha-L-Rha Beta-L-Rha Alpha-L-Rha-(1->3)-Alpha-D-GlcNAc Escherichia coli
N
NO
Alpha-L-Rha Alpha-L-Rha Alpha-L-Rha-(1->3)-Alpha-D-GlcNAc Escherichia coli
N
NO
Alpha-L-Rha Alpha-D-GlcNAc Alpha-L-Rha-(1->3)-Alpha-D-GlcNAc Escherichia coli
N
NO
Alpha-L-Rha Alpha-L-FucNAc Alpha-L-Rha-(1->4)-Alpha-L-FucNAc Escherichia coli
N
NO
Beta-L-Rha Beta-D-GlcNAc Beta-L-Rha-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Beta-L-Rha Beta-L-Rha Beta-L-Rha-(1->4)-Beta-L-Rha Escherichia coli
N
NO
Beta-L-Rha Beta-D-GlcNAc Beta-L-Rha-(1->4)-Beta-D-GlcNAc Escherichia coli
N
NO
Beta-L-Rha Alpha-D-GlcNAc Beta-L-Rha-(1->4)-Alpha-D-GlcNAc Escherichia coli
Y
NO
Alpha-D-Man Beta-D-GlcNAc Alpha-D-Man-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Alpha-D-Man Alpha-D-Man Alpha-D-Man-(1->3)-Alpha-D-Man Escherichia coli
N
NO
Alpha-D-Man Beta-D-Gal Alpha-D-Man-(1->4)-Beta-D-Gal Escherichia coli
N
NO
Alpha-D-Man Alpha-D-Man Alpha-D-Man-(1->2)-Alpha-D-Man Escherichia coli
N
NO
Beta-D-Man Alpha-D-GlcNAc Beta-D-Man-(1->3)-Alpha-D-GlcNAc Escherichia coli
N
NO
Beta-D-Man Beta-D-Gal Beta-D-Man-(1->3)-Beta-D-Gal Escherichia coli
N
NO
Alpha-D-Galf Beta-D-GalA Alpha-D-Galf-(1->4)-Beta-D-GalA Shigella boydii
Y
NO
Beta-D-Galf Alpha-D-Glc Beta-D-Galf-(1->6)-Alpha-D-Glc Escherichia coli
N
NO
Beta-D-Galf Beta-D-Galf Beta-D-Galf-(1->5)-Beta-D-Galf Shigella boydii
N
NO
Beta-D-Galf Beta-D-GlcNAc Beta-D-Galf-(1->3)-Beta-D-GlcNAc Shigella boydii
N
NO
Beta-D-Galf Beta-D-GlcNAc Beta-D-Galf-(1->3)-Beta-D-GlcNAc Shigella boydii
N
NO
Beta-D-Galf Beta-D-GalNAc Beta-D-Galf-(1->3)-Beta-D-GalNAc Escherichia coli
N
NO
Alpha-D-Glc Alpha-D-Glc Alpha-D-Glc-(1->4)-Alpha-D-Glc Escherichia coli
N
NO
Alpha-D-Glc Alpha-L-Rha Alpha-D-Glc-(1->3)-Alpha-L-Rha Escherichia coli
N
NO
Alpha-D-Glc Alpha-L-FucNAc Alpha-D-Glc-(1->3)-Alpha-L-FucNAc Escherichia coli
N
NO
Alpha-D-Glc Beta-D-Glc Alpha-D-Glc-(1->2)-Beta-D-Glc Escherichia coli
N
NO
Alpha-D-Glc Alpha-D-Glu Alpha-D-Glc-(1->4)-Alpha-D-Glc-(1->4)-1,4-Alpha-D-Glu Escherichia coli
N
NO
Beta-D-Glc Beta-D-GalNAc Beta-D-Glc-(1->3)-Beta-D-GalNAc Escherichia coli
N
NO
Beta-D-Glc Alpha-D-GalNAc Beta-D-Glc-(1->3)-Alpha-D-GalNAc Escherichia coli
N
NO
Beta-D-Glc Beta-D-GlcNAc Beta-D-Glc-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Beta-D-GlcA Alpha-D-GlcNAc Beta-D-GlcA-(1->4)-Alpha-D-GlcNAc Escherichia coli
N
NO
Beta-D-GlcA Alpha-D-Man Beta-D-GlcA-(1->2)-Alpha-D-Man Escherichia coli
N
NO
Alpha-D-GlcNAc Und-P Alpha-D-GlcNAc-P-P-Und Escherichia coli
N
NO
Alpha-D-GlcNAc Beta-D-Galf Alpha-D-GlcNAc-(1->2)-Beta-D-Galf Shigella boydii
N
NO
Alpha-D-GlcNAc Alpha-D-GalA Beta-D-GlcNAc-(1->4)-Alpha-D-GalA Shigella dysenteriae
N
NO
Alpha-D-GlcNAc Alpha-D-GlcA Alpha-D-GlcNAc-(1->4)-Alpha-DGlcA Escherichia coli
N
NO
Alpha-D-GlcNAc Alpha-D-Glc Alpha-D-GlcNAc-(1->6)-Alpha-D-Glc Escherichia coli
N
NO
Alpha-D-GlcNAc Beta-D-GlcNAc Alpha-D-GlcNAc-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Beta-D-GlcNAc Alpha-L-Rha Beta-D-GlcNAc-(1->2)-Alpha-L-Rha Escherichia coli
N
NO
Beta-D-GlcNAc Beta-D-Glc Beta-D-GlcNAc-(1->2)-Beta-D-Glc Escherichia coli
Y
NO
Alpha-Neu5Ac Beta-D-GlcNAc Alpha-Neu5Ac-(2->6)-Beta-D-Gal-(1->4)-Beta-D-GlcNAc Drosophila spp.
N
NO
Beta-D-Glc Beta-D-GlcNAc Beta-D-Glc-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Alpha-D-Gal Beta-D-Glc Alpha-D-Gal-(1->2)-Beta-D-Glc Escherichia coli
Y
NO
Alpha-Neu5Ac Beta-D-Glc Alpha-Neu5Ac-(2->3)-Beta-D-Glc Escherichia coli
N
NO
Alpha-D-Gal Beta-D-GlcNAc Alpha-D-Gal-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Beta-D-Gal Beta-D-GlcNAc Beta-D-Gal-(1->3)-Beta-D-GlcNAc Escherichia coli
Y
NO
Beta-D-GlcNAc Alpha-D-Gal Beta-D-GlcNAc-(1->3)-Alpha-D-Gal Escherichia coli
N
NO
Alpha-L-6dTal Alpha-D-FucNAc Alpha-L-6dTal-(1->3)-Alpha-D-FucNAc Escherichia coli
N
NO
Beta-D-Glc Alpha-L-6dTal Beta-D-Glc-(1->3)-Alpha-L-6dTal Escherichia coli
N
NO
Alpha-D-Gal Beta-D-GlcNAc Alpha-D-Gal-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Beta-D-Gal Beta-D-Man Beta-D-Gal-(1->4)-Beta-D-Man Escherichia coli
N
NO
Beta-D-Man Alpha-D-Gal Beta-D-Man-(1->4)-Alpha-D-Gal Escherichia coli
N
NO
Alpha-D-Glc Alpha-D-Gal Alpha-D-Glc-(1->6)-Alpha-D-Gal Shigella dysenteriae
N
NO
Alpha-D-Gal Alpha-L-FucNAc Alpha-D-Gal-(1->3)-Alpha-L-FucNAc Shigella dysenteriae
Y
NO
Beta-D-Glc Alpha-L-FucNAc Beta-D-Glc-(1->4)-Alpha-L-FucNAc Shigella dysenteriae
N
NO
Beta-D-GlcNAc Beta-D-Galf Beta-D-GlcNAc-(1->3)-Beta-D-Galf Escherichia coli
N
NO
Beta-D-Galf Alpha-D-GlcNAc Beta-D-Galf-(1->3)-Alpha-D-GlcNAc Escherichia coli
N
NO
Alpha-D-Man Alpha-D-Man Alpha-D-Man-(1->4)-Alpha-D-Man Escherichia coli
N
NO
Alpha-D-Man Beta-D-GlcNAc Alpha-D-Man-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Alpha-D-GlcNAc Beta-D-Man Alpha-D-GlcNAc-(1->2)-Beta-D-Man Escherichia coli
N
NO
Beta-D-Glc Alpha-L-6dTal Beta-D-Glc-(1->3)-Alpha-L-6dTal Escherichia coli
N
NO
Alpha-D-Man Beta-D-Man Alpha-D-Man-(1->2)-Beta-D-Man Escherichia coli
N
NO
Alpha-D-Man Alpha-D-Man Alpha-D-Man-(1->2)-Alpha-D-Man Escherichia coli
N
NO
Beta-D-Man Alpha-D-GlcNAc Beta-D-Man-(1->3)-Alpha-D-GlcNAc Escherichia coli
N
NO
Alpha-D-Gal Beta-D-Gal Alpha-D-Gal-(1->3)-Beta-D-Gal Escherichia coli
Y
NO
Beta-D-Gal Alpha-D-GalNAc Beta-D-Gal-(1->3)-Alpha-D-GalNAc Escherichia coli
N
NO
Beta-D-Gal Beta-D-GalNAc Beta-D-Gal-(1->3)-Beta-D-GalNAc Escherichia coli
N
NO
Alpha-D-GalNAc Beta-D-GalNAc Alpha-D-GalNAc-(1->3)-Beta-D-GalNAc Escherichia coli
N
NO
Beta-D-Gal Beta-D-GlcNAc Beta-D-Gal-(1->4)-Beta-D-GlcNAc Escherichia coli
N
NO
Alpha-L-QuiNAc Alpha-D-GalNAcA Alpha-L-QuiNAc-(1->4)-Alpha-D-GalNAcA Escherichia coli
N
NO
Alpha-D-GalNAcA Alpha-L-QuiNAc Alpha-D-GalNAcA-(1->3)-Alpha-L-QuiNAc Escherichia coli
N
NO
Alpha-L-QuiNAc Beta-D-GlcNAc Alpha-L-QuiNAc-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Alpha-D-Rha Alpha-D-Rha Alpha-D-Rha-(1->2)-Alpha-D-Rha Escherichia coli
N
NO
Alpha-D-Rha Alpha-D-Rha Alpha-D-Rha-(1->3)-Alpha-D-Rha Escherichia coli
N
NO
Alpha-Neu5Ac Beta-D-Gal Alpha-D-Neu5Ac-(2->3)-Beta-D-Gal Escherichia coli
N
NO
Alpha-D-Gal Alpha-Neu5Ac Alpha-D-Gal-(1->4)-Alpha-Neu5Ac Escherichia coli
N
NO
Beta-D-Gal Beta-D-GalNAc Beta-D-Gal-(1->3)-Beta-D-GalNAc Escherichia coli
N
NO
Beta-D-Ribf Alpha-L-Rha Beta-D-Ribf-(1->2)-Alpha-L-Rha Escherichia coli
Y
NO
Beta-D-GalNAc Alpha-L-Rha Beta-D-GalNAc-(1->3)-Alpha-L-Rha Escherichia coli
N
NO
Alpha-L-Rha Alpha-D-GlcNAc Alpha-L-Rha-(1->4)-Alpha-D-GlcNAc Escherichia coli
N
NO
Alpha-D-GlcNAc Beta-D-Gal Alpha-D-GlcNAc-(1->4)-Beta-D-Gal Escherichia coli
N
NO
Beta-D-Gal Alpha-D-GalNAc Beta-D-Gal-(1->3)-Alpha-D-GalNAc Escherichia coli
N
NO
Alpha-D-Gal Beta-D-GlcNAc Alpha-D-Gal-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Alpha-Col Alpha-D-Glc Alpha-Col-(1->3)-Alpha-D-Glc OR Alpha-Col-(1->6)-Alpha-D-Glc Escherichia coli
Y
NO
Beta-D-GlcNAc Alpha-D-GalNAc Beta-D-GlcNAc-(1->3)-Alpha-D-GalNAc Escherichia coli
Y
NO
Alpha-D-GalNAc Alpha-D-Glc Alpha-D-GalNAc-(1->4)-Alpha-D-Glc Shigella boydii
N
NO
Beta-D-Gal Alpha-D-GalNAc Beta-D-Gal-(1->3)-Alpha-D-GalNAc Escherichia coli
Y
NO
Alpha-D-GalA Alpha-D-Gal Alpha-D-GalA-(1->3)-Alpha-D-Gal Escherichia coli
N
NO
Alpha-D-GalNAc Alpha-D-GalA Alpha-D-GalNAc-(1->4)-Alpha-D-GalA Escherichia coli
N
NO
Alpha-D-Gal Beta-D-GlcNAc Alpha-D-Gal-(1->3)-Beta-D-GlcNAc Escherichia coli
N
NO
Beta-D-Ribf Beta-D-Gal Beta-D-Ribf-(1->4)-Beta-D-Gal Escherichia coli
N
NO
Beta-D-Gal Alpha-D-GlcNAc Beta-D-Gal-(1->3)-Alpha-D-GlcNAc Escherichia coli
N
NO
Beta-D-GalNAc Alpha-L-Rha Beta-D-GalNAc-(1->3)-Alpha-L-Rha Escherichia coli
N
NO
Alpha-L-Rha Alpha-D-Glc Alpha-L-Rha-(1->4)-Alpha-D-Glc Escherichia coli
N
NO
Alpha-D-Glc Beta-D-Gal Alpha-D-Glc-(1->4)-Beta-D-Gal Escherichia coli
N
NO
Beta-D-Gal Alpha-D-GalNAc Beta-D-Gal-(1->3)-Alpha-D-GalNAc Escherichia coli
N
NO
Alpha-D-Glc Alpha-D-Gal Alpha-D-Glc-(1->2)-Alpha-D-Gal Escherichia coli
N
NO
Alpha-D-Glc Alpha-D-Glc Alpha-D-Glc-(1->2)-Alpha-D-Glc Escherichia coli
N
NO
Alpha-D-Gal Alpha-D-Glc Alpha-D-Gal-(1->2)-Alpha-D-Glc Escherichia coli
N
NO
Alpha-D-Glc Alpha-D-Glc Alpha-D-Glc-(1->3)-Alpha-D-Glc Escherichia coli
N
NO
Alpha-D-Gal Alpha-D-Gal Alpha-D-Gal-(1->2)-Alpha-D-Gal Escherichia coli
Y
NO
Beta-D-Glc Alpha-D-Glc Beta-D-Glc-(1->3)-Alpha-D-Glc Escherichia coli
Y
NO
Alpha-D-Gal Alpha-D-Glc Alpha-D-Gal-(1->6)-Alpha-D-Glc Escherichia coli
Y
NO
Alpha-D-GlcNAc Alpha-D-Glc Alpha-D-GlcNAc-(1->2)-Alpha-D-Glc Escherichia coli
Y
NO
Alpha-D-Glc Alpha-D-Glc Alpha-D-Glc-(1->2)-Alpha-D-Glc Escherichia coli
Y
NO
Alpha-D-Gal Alpha-D-Glc Alpha-D-Gal-(1->3)-Alpha-D-Glc Escherichia coli
N
NO
Alpha-D-GalNAc Alpha-D-GalNAc Alpha-D-GalNAc-(1->3)-Alpha-D-GalNAc Escherichia coli
N
YES
Beta-D-Gal Alpha-D-GalNAc Beta-D-Gal-(1->3)-Alpha-D-GalNAc Escherichia coli
N
YES
Alpha-L-Fuc Beta-D-Gal Alpha-L-Fuc-(1->2)-Beta-D-Gal Escherichia coli
Y
YES
Alpha-D-Gal Beta-D-Gal Alpha-D-Gal-(1->3)-Beta-D-Gal Escherichia coli
N
YES
Beta-D-GalNAc Alpha-D-Gal Beta-D-GalNAc-(1->4)-Alpha-D-Gal Escherichia coli
N
YES