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Team:UT-Tokyo/LabWork/RegularMethods
From 2012.igem.org
(Difference between revisions)
| Line 23: | Line 23: | ||
====Protocol==== | ====Protocol==== | ||
| - | + | =====1.Make reaction liquid===== | |
| - | + | *MilliQ up to 20uL | |
| - | + | *10uL 2x Ligation Mix | |
| - | + | *Vector DNA | |
| - | + | *Insert DNA | |
| - | + | =====2.Incubation at 16 °C for 15-30 min.===== | |
<!-- 以上自由記述 --> | <!-- 以上自由記述 --> | ||
Revision as of 21:41, 26 September 2012
Regular Methods
Assembly parts
Digest
Materials
Protocol
Ligation
Materials
- Vector DNA
- Insert DNA
- 2x Ligation Mix
Protocol
1.Make reaction liquid
- MilliQ up to 20uL
- 10uL 2x Ligation Mix
- Vector DNA
- Insert DNA
2.Incubation at 16 °C for 15-30 min.
