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Team:UT-Tokyo/LabWork/RegularMethods
From 2012.igem.org
(Difference between revisions)
| Line 24: | Line 24: | ||
====Protocol==== | ====Protocol==== | ||
*1.Make reaction liquid | *1.Make reaction liquid | ||
| - | + | MilliQ up to 20uL | |
| - | + | 10uL 2x Ligation Mix | |
| - | + | Vector DNA | |
| - | + | Insert DNA | |
*2.Incubation at 16 °C for 15-30 min. | *2.Incubation at 16 °C for 15-30 min. | ||
Revision as of 21:40, 26 September 2012
Regular Methods
Assembly parts
Digest
Materials
Protocol
Ligation
Materials
- Vector DNA
- Insert DNA
- 2x Ligation Mix
Protocol
- 1.Make reaction liquid
MilliQ up to 20uL 10uL 2x Ligation Mix Vector DNA Insert DNA
- 2.Incubation at 16 °C for 15-30 min.
