Team:LMU-Munich/Data

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Revision as of 21:28, 26 September 2012

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

[ more news ]

Data banner.jpg

Data

Here you will find all of our project data.

BacillusBioBrickBox

Evaluation of B. subtilis vectors


Seven novel vectors were constructed and four already proven to work in B. subtilis

Anderson Promoter Evaluation


Eleven Anderson promoters were measured in B. subtilis and showed relatively weak activity.

Constitutive Bacillus Promoters


Three novel B. subtilis promoter BioBricks (P_liaG, P_veg, P_lepA) were constructed and functionality verified by luminescence and β-galactosidase assays.

Inducible Bacillus Promoters


A novel antibiotic-inducible promoter,P_liaI, was constructed and carefully evaluated.

Sporobeads

Crust Promoter Evaluation


Three sporulation-dependent promoters (blablabla) were constructed, two of which show the expected behavior.

GFP-Sporobead Evaluation


They glow! GFP was successfully displayed on the spore crust as demonstreted by fluorescence microscopy

Sporobead Purification


We compared different ways of purifying Bacillus spores from vegetative cells: Lysosome is the best!

GerminationSTOP

Knockouts of germination genes


We successfully prevented spore germination by multiple gene knockouts

Suicide Switch


Death upon germination: We developed a novel safety strategy as a back-up to knockouts.

Inverter

β-galactosidase assay of Inverter with lacZα


β-galactosidase assay of Inverter with lacZα dependent on Arabinose concentrations

Trashcan

Things that were a pain in the neck!

cgeA and P_cgeA


Unexpected restriction sites combined with no activity at all

MazF

The story of trying to clone a toxine from E. coli in E. coli

The story of trying to clone a toxine from E. coli in E. coli

Xylose-promoters

All constructs involving Xylose-inducible promoter refused cloning or evaluation.

All constructs involving Xylose-inducible promoter refused cloning or evaluation.

For our big breakthroughs, or to follow specific projects, see the individual project pages:

Project Navigation


Bacillus Intro	Bacillus BioBrickBox	Sporobeads	Germination STOP

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Data"

@@ Line 61: / Line 61: @@
 ===Crust Promoter Evaluation===
 {| "width=100%" style="text-align:center;"|
-|<p align="justify">We evaluated the three promoters for the crust proteins</p>
+|<p align="justify">Three sporulation-dependent promoters (blablabla) were constructed, two of which show the expected behavior.</p>
 |[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]]
 |-
@@ Line 71: / Line 71: @@
 ===GFP-'''Sporo'''bead Evaluation===
 {| "width=100%" style="text-align:center;"|
-|<p align="justify">We fused a GFP to the spore crust with five different constructs in two different genetic ''B. subtilis'' strains and analysed the strength with fluorescence microscopy</p>
+|<p align="justify">They glow! GFP was successfully displayed on the spore crust as demonstreted by fluorescence microscopy</p>
 |[[File:Sporobead Constructs Dada sheet preview.png|200px|link=Team:LMU-Munich/Data/gfp_spore]]
 |-
@@ Line 81: / Line 81: @@
 ==='''Sporo'''bead Purification===
 {| "width=100%" style="text-align:center;"|
-|<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells</p>
+|<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells: Lysosome is the best!</p>
 |[[File:Purification preview.png|200px|link=Team:LMU-Munich/Data/Sporepurification]]
 |-
@@ Line 99: / Line 99: @@
 ===Knockouts of germination genes ===
 {| "width=100%" style="text-align:center;"|
-|<p align="justify">Germination assy of ''Bacillus'' strains with multiple gene knockouts</p>
+|<p align="justify">We successfully prevented spore germination by multiple gene knockouts</p>
 |[[File:LMU Knockout previewii.jpg|200px|link=Team:LMU-Munich/Data/Knockout]]
 |-
@@ Line 108: / Line 108: @@
 ===Suicide Switch ===
 {| "width=100%" style="text-align:center;"|
-|<p align="justify">Luminescence assay of <b>Suicide</b> switch with ''luxABCDE'' in the place of MazF with P<sub>''spoIVB''</sub></p>
+|<p align="justify">Death upon germination: We developed a novel safety strategy as a back-up to knockouts.</p>
 |[[File:PlatereaderSuicideSwitch1.jpg|200px|link=Team:LMU-Munich/Data/Suicideswitch]]
 |-