Team:Exeter/lab book/1gp/wk7

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         <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk7"; style="color:#1d1d1b">20th - 24th August</a>
         <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk7"; style="color:#1d1d1b">20th - 24th August</a>
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        <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk8"; style="color:#1d1d1b">27th - 31st August</a>
 
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        <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk9"; style="color:#1d1d1b">3rd - 7th September</a>
 
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        <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk10"; style="color:#1d1d1b">10th - 14th September</a>
 
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Revision as of 20:53, 26 September 2012

ExiGEM2012 Lab Book 1GP wk7

Single Gene Plasmids and Enzyme Characterisation: 20th - 24th August 2012

Monday 20th August (11.00)

• 3A assembly of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3

Tuesday 21st August (9.00)

• Transformation of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3

Wednesday 22nd August (9.00)

• 3A assembly of WclY-pSB1C3 and WbiP-pSB1C3

• Adding cultures with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight

No colonies with ligated WclY-pSB1C3 and WbiP-pSB1C3 were found and thus 3A assembly was repeated.

Thursday 23rd August (9.00)

• Adding cultures with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight

• Transformation of WclY-pSB1C3 and WbiP-pSB1C3

The shaking incubator broke on Wednesday evening and therefore adding cultures into liquid medium was repeated.

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