Team:Westminster/Experiments

From 2012.igem.org

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<h2>Lorem Ipsum</h2>
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<h1>BIOINFORMATICS</h1>
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<p>Cancer recurrence is one of the fears that almost every patient undergoing chemotherapy develops. Recent findings suggest that only a small fraction of the tumor cells, called Cancer Stem Cells (CSC) are able to drive the growth of the tumor. CSC also show an increased drug resistance, and could remain unaffected after chemotherapy, eventually resulting in the formation of a new tumor.</p>
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<h2>Promoter Seqeunce Identification</h2>
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<p>Cancer recurrence is one of the fears that almost every patient undergoing chemotherapy develops. Recent findings suggest that only a small fraction of the tumor cells, called Cancer Stem Cells (CSC) are able to drive the growth of the tumor. CSC also show an increased drug resistance, and could remain unaffected after chemotherapy, eventually resulting in the formation of a new tumor.</p>
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<p>The team isolated regions of approximately 1kbp where the gene promoter was likely to be found. These were compared with commercial promoters (if available) and European Promoter database (EPD). The promoter sequences that appeared on the EPD site have been experimentally analysed, and also did not contain any illegal sites. Thus they were selected, in case of ADH1A1 and ALDH3A1. ALDH1A3 identified by the team had no illegal site, so it was maintained. However, ALDH2 promoter sequence on the EPD site was too small to be included. Therefore a commercial promoter of ALDH2 was selected.</p>
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<img src="https://static.igem.org/mediawiki/2012/4/4d/Westminster_team.png" alt="Welcome" title="Welcome to our home!" width="885" height="220" />
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<p>The following gives an example of how a promoter sequences were identified by the iSTEM team:</p>
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<h2>ALDH1A1</h2>
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<img src="https://static.igem.org/mediawiki/2012/6/6e/Box_a.png" alt="ALDH1A1 directions" title="1A1" width="885" height="220" />
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Revision as of 20:29, 26 September 2012

Welcome

BIOINFORMATICS

Promoter Seqeunce Identification

The team isolated regions of approximately 1kbp where the gene promoter was likely to be found. These were compared with commercial promoters (if available) and European Promoter database (EPD). The promoter sequences that appeared on the EPD site have been experimentally analysed, and also did not contain any illegal sites. Thus they were selected, in case of ADH1A1 and ALDH3A1. ALDH1A3 identified by the team had no illegal site, so it was maintained. However, ALDH2 promoter sequence on the EPD site was too small to be included. Therefore a commercial promoter of ALDH2 was selected.

The following gives an example of how a promoter sequences were identified by the iSTEM team:

ALDH1A1

ALDH1A1 directions

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