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Team:University College London/Protocols/T4dnaligase
From 2012.igem.org
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Revision as of 20:15, 26 September 2012
Transformation Protocol 2
Nuclease positive cells
| COMPONENT | 20 μl REACTION) |
|---|---|
| 10X T4 DNA Ligase Buffer | 2 μl |
| Vector DNA (3 kb) | 50 ng (0.025 pmol) |
| Insert DNA (1 kb | 50 ng (0.076 pmol) |
| Nuclease-free water | to 20 μl |
| T4 DNA Ligase | 1 μl |
