"
Team:University College London/Protocols/T4dnaligase
From 2012.igem.org
(Difference between revisions)
Aurelija (Talk | contribs)
(Created page with "== Transformation Protocol 2 == </noinclude> Nuclease positive cells {| class="bigtable" |- ! COMPONENT !! 20 μl REACTION) |- | 10X T4 DNA Ligase Buffer || 2 μl |- | Vector ...")
Newer edit →
(Created page with "== Transformation Protocol 2 == </noinclude> Nuclease positive cells {| class="bigtable" |- ! COMPONENT !! 20 μl REACTION) |- | 10X T4 DNA Ligase Buffer || 2 μl |- | Vector ...")
Newer edit →
Revision as of 20:15, 26 September 2012
Transformation Protocol 2
Nuclease positive cells
| COMPONENT | 20 μl REACTION) |
|---|---|
| 10X T4 DNA Ligase Buffer | 2 μl |
| Vector DNA (3 kb) | 50 ng (0.025 pmol) |
| Insert DNA (1 kb | 50 ng (0.076 pmol) |
| Nuclease-free water | to 20 μl |
| T4 DNA Ligase | 1 μl |
