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Team:Groningen/Construct
From 2012.igem.org
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| - | Our construct idea is simple and effective: there will be a production of pigment under the regulation of rotten-meat reactive promoter. When | + | Our construct idea is simple and effective: there will be a production of pigment under the regulation of a rotten-meat reactive promoter. When <i>Bacillus subtilis</i> senses the volatiles from the rotten meat, the rotten meat promoter becomes active thus allowing the production of downstream genes. We placed pigment genes under the control of the promoter so that the pigment would be produced when the promoter is activated.<br> |
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| - | We use our <i>Bacillus subtilis</i> backbone (BBa_K818000) that has <i>sacA</i> and chloramphenicol resistance gene for chromosomal integration and | + | We use our <i>Bacillus subtilis</i> backbone (BBa_K818000) that has <i>sacA</i> and a chloramphenicol resistance gene for chromosomal integration and antibiotic screening of transformants. This backbone also has <i>E. coli</i> origin of replication, so it can be amplified inside <i>E. coli</i>. |
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Revision as of 19:05, 26 September 2012
Our construct idea is simple and effective: there will be a production of pigment under the regulation of a rotten-meat reactive promoter. When Bacillus subtilis senses the volatiles from the rotten meat, the rotten meat promoter becomes active thus allowing the production of downstream genes. We placed pigment genes under the control of the promoter so that the pigment would be produced when the promoter is activated.
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We use our Bacillus subtilis backbone (BBa_K818000) that has sacA and a chloramphenicol resistance gene for chromosomal integration and antibiotic screening of transformants. This backbone also has E. coli origin of replication, so it can be amplified inside E. coli.
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