Team:Paris-Saclay/Project/Notebook/Week 9

From 2012.igem.org

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[[Category:Team:Paris-Saclay/Project Gemote/Notebook|i]]
[[Category:Team:Paris-Saclay/Project Gemote/Notebook|i]]
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====30th july====
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*Analysis of the Petri dishes after the transformation with the B construction
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{| width="85%"
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|-
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| style="width: 50%;"|Electrophoresis of BBa_K274100 and pSB1A2 on a 0.8% Agarose gel.
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| style="width: 35%;"| [[File:Week9-1.jpg|right|400px]]
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|}
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====31th July====
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*Miniprep of the colonies B1 and B2 that came from the transformation with the B construction.
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*Nanovue to measure the concentration of the samples B1, B2 and the control
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[[File:Week9-2.jpg|400px]]
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*Amplification by PCR of the 880bp end of BBa_K098995 contained in the colonies B1, B2 and test on the control and amplification of the whole B construction.
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[[File:Week9-3.jpg|400px]]
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PCR program used:
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[[File:Week9-4.jpg|400px]]
{{Team:Paris-Saclay/Follow}}
{{Team:Paris-Saclay/Follow}}
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{{Team:Paris-Saclay/Footer}}
{{Team:Paris-Saclay/Footer}}

Revision as of 18:54, 26 September 2012

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30th july

  • Analysis of the Petri dishes after the transformation with the B construction
Electrophoresis of BBa_K274100 and pSB1A2 on a 0.8% Agarose gel.
Week9-1.jpg


31th July

  • Miniprep of the colonies B1 and B2 that came from the transformation with the B construction.
  • Nanovue to measure the concentration of the samples B1, B2 and the control

Week9-2.jpg

  • Amplification by PCR of the 880bp end of BBa_K098995 contained in the colonies B1, B2 and test on the control and amplification of the whole B construction.

Week9-3.jpg

PCR program used:

Week9-4.jpg

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