Team:UC Chile/Cyano/Notepad/week16

From 2012.igem.org

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<ul>Quest to destroy the ring :
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<ul>Quest to destroy the ring :  
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<li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad">week 1</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week6">week 6</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week7">week 7</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week8">week 8</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week9">week 9</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week10">week 10</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week11">week 11</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week12">week 12</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week13">week 13</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week14">week 14</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week15">week 15</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week16">week 16</a></li>
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<li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week1">w 1</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week2">w 2</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week3">w 3</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week4">w 4</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week5">w 5</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week6">w 6</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week7">w 7</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week8">w 8</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week9">w 9</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week10">w 10</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week11">w 11</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week12">w 12</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week13">w 13</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week14">w 14</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week15">w 15</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week16">w 16</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week17">w 17</a></li>
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<p><br><br><b>Tuesday:</b> Tried Gibson with new enzymes (again). KanR was amplified by PCR (finally after four failed attempts!!).
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Also, we invited Dr. Endy to our Synbio Workshop in December and he kindly said he'd come (yeah!).<br><br><br>
 
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<b>Wednesday:</b> Gibson did not work this time so now we believe the temperature is the cause (we keep the DNA in ice throughout the whole procedure until the heat shock). This reaction worked in summer. Winter has come nad we ar ewaaaaaaay beyond the wall.
 
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Tried Gibson at different room temperature exposure times before heat shcok: no exposure time, 5 seconds, 15 seconds and room temperature.<br><br><br>
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== Week 16 ==
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<b>Thursday:</b><br><br>
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Results of previous experiment:<br><br>
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- no exposure = +<br>
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- room temperature = +<br>
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- 5 sec = -<br>
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- 15 sec = -<br><br>
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Conclusion: We have extremists bacteria!!!<br><br>
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Well, did one more Gibson at room temperature... waiting for results.<br><br>
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Just got off the phone with Fernán (the Argentinian).
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He says: "Our Gibsons haven't worked lately, I think that the cells are lacking... The are only 10´9 colonies per ug of PUC19 DNA."
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10´9? Thats just 10 to 50 more times than ours! ... Starting Knight's TOP10 Competent protocol again (3 time this year)!
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Revision as of 22:23, 28 June 2012

Cyanolux & SpiderColi - Pontificia Universidad Católica de Chile, iGEM 2012



notebook


Week 16

Retrieved from "http://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week16"