"
Team:Paris-Saclay/Project/Notebook/Week 7
From 2012.igem.org
(Difference between revisions)
YohannPetiot (Talk | contribs) |
YohannPetiot (Talk | contribs) |
||
| Line 52: | Line 52: | ||
[[Category:Team:Paris-Saclay/Project Gemote/Notebook|g]] | [[Category:Team:Paris-Saclay/Project Gemote/Notebook|g]] | ||
__NOTOC__ | __NOTOC__ | ||
| + | *PCR in large volume to have a lot a available biobricks | ||
| + | *Treatment with DPN1 to eliminate plasmids | ||
| + | *Gel purification to only keep our interest fragments | ||
| + | *Gibson Assembly method to put together the different biobricks for each construction | ||
| + | *Transformation with assembly products and staggering on a selective medium | ||
| + | *Colony PCR to have results before the transformation results | ||
| + | |||
{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
Revision as of 16:43, 26 September 2012
- PCR in large volume to have a lot a available biobricks
- Treatment with DPN1 to eliminate plasmids
- Gel purification to only keep our interest fragments
- Gibson Assembly method to put together the different biobricks for each construction
- Transformation with assembly products and staggering on a selective medium
- Colony PCR to have results before the transformation results
Follow us !