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Team:Paris-Saclay/Project/Notebook/Week 4
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*Miniprep to purify each biobrick (ready to use) | *Miniprep to purify each biobrick (ready to use) | ||
*Groove for two colonies because they did not grown on the liquid culture | *Groove for two colonies because they did not grown on the liquid culture | ||
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{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
Revision as of 16:40, 26 September 2012
- We place an order for the primers
- DNA (biobricks) Resuspension using iGEM protocol
- Transformation of each biobricks by thermal shock to have a large quantity of DNA
- Staggering of the transformed bacteria on a selective medium to select bacteria which received the plasmid
- We put some colonies, for each biobricks, on liquid culture to be able to make a Miniprep
- Miniprep to purify each biobrick (ready to use)
- Groove for two colonies because they did not grown on the liquid culture
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