(Difference between revisions)

Revision as of 15:46, 26 September 2012

BioBrick

		Name	Type	Description	Designer	Length
1	W	BBa_K821001	Reporter	RFP Reporter with o-RBS	Qinfeng Wu, Bin Jia	1059
2	W	BBa_K821002	Coding	Orthogonal 16S DNA sequence from rrnB	Qinfeng Wu, Bin Jia	1894
3	W	BBa_K821012	Generator	PBad(promoter)+orthogonal 16S RNA	Jinlai Zhang, Dongchang Qin	3226

		Name	Type	Description	Designer	Length
1	W	BBa_K821003	Coding	16S DNA sequence from rrnB	Jinlai Zhang, Dongchang Qin	1894
2	W	BBa_K821004	Regulatory	PBad Promoter	Jinlai Zhang, Dongchang Qin	1324
3	W	BBa_K821005	Reporter	CmR label from pKD3	Qinfeng Wu, Bin Jia	1014
4	W	BBa_K821006	Project	KanR label from pKD3	Jinlai Zhang, Dongchang Qin	1304
5	W	BBa_K821007	Composite	PL(promoter)+orthogonal 16S RNA	Qinfeng Wu, Bin Jia	1957
6	W	BBa_K821008	Composite	PLtet+orthogonal 16S RNA	Jinlai Zhang, Dongchang Qin	1956
7	W	BBa_K821009	RBS	Orthogonal RBS 1	Qinfeng Wu, Bin Jia	6
8	W	BBa_K821010	Composite	PLacI+Native RBS+RFP+Native RBS+GFP+Terminator	Qinfeng Wu, Bin Jia	1833
9	W	BBa_K821003	Composite	PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator	Jinlai Zhang, Dongchang Qin	1827

7.14 t7 rbs' crtE

7.31 16s' RFP-GFP AMP

8.03 pSB1C3 K3 Digestion

8.16 16s' mutate to remove EcoRI

8.17 16S' pSB1C3

8.17 Mutate the RBS of Amp using PYES2

8.17 Yeast Assembly using pSB3C5R & pSB3K3

8.18 pBad-16S' remove EcorI, PSB1C3

9.2 6I 6G Gel extraction and verification

9.2 6I,6G P16S Gel extraction and verification

9.2 P16S digestion

9.16 yeast cPCR 1

9.16 yeast cPCR 2

81 16s GFP & RFP

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+<tr>
+<th style="background:#f680c4;">&nbsp;</th>
+<th style="background:#f680c4;">&nbsp;</th>
+<th style="background:#f680c4;">Name</th>
+<th style="background:#f680c4;">Type</th>
+<th style="background:#f680c4;">Description</th>
+<th style="background:#f680c4;">Designer</th>
+<th style="background:#f680c4;">Length</th>
+</tr>
 <tr>