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File:Tokyotech PHA biobrick.png
From 2012.igem.org
((({{Information| |Description= To construct a part that meets Biobrick format, we have modified the pha-C1-A-B1 operon not to contain forbidden restriction enzyme sites. First, we cloned the wild type gene pha-C1-A-B1 from R.eutropha H16 by using PCR and ) |
(uploaded a new version of "File:Tokyotech PHA biobrick.png") |
Latest revision as of 15:45, 26 September 2012
(({{Information| |Description= To construct a part that meets Biobrick format, we have modified the pha-C1-A-B1 operon not to contain forbidden restriction enzyme sites. First, we cloned the wild type gene pha-C1-A-B1 from R.eutropha H16 by using PCR and inserted the gene into pSB1C3. However, wild type pha-C1-A-B1 gene sequence contains one NotI and three PstI recognition sites that are not allowed in Biobrick format. To get pha-C1-A-B1 sequence without these recognition sites, we ordered the chemically synthesized DNA from IDT/MBL. In this chemically synthesized DNA, coding is optimized for E.coli. That is to say, we got PHB synthesizing gene in Biobrick format (BBa_K934001).
|Source=Takuo's file |Date=2012/09/20 |Author=Taku nakayama ))
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 15:45, 26 September 2012 |  | 926×877 (73 KB) | Yasuo (Talk | contribs) | |
| 15:46, 22 September 2012 |  | 564×579 (75 KB) | Takuo (Talk | contribs) | ((({{Information| |Description= To construct a part that meets Biobrick format, we have modified the pha-C1-A-B1 operon not to contain forbidden restriction enzyme sites. First, we cloned the wild type gene pha-C1-A-B1 from R.eutropha H16 by using PCR and ) |
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