From 2012.igem.org

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Revision as of 15:44, 26 September 2012

BioBrick

Favorite Tianjin 2012 iGEM Team Parts

		Name	Type	Description	Designer	Length
1	W	BBa_K821001	Reporter	RFP Reporter with o-RBS	Qinfeng Wu, Bin Jia	1059
2	W	BBa_K821002	Coding	Orthogonal 16S DNA sequence from rrnB	Qinfeng Wu, Bin Jia	1894
3	W	BBa_K821012	Generator	PBad(promoter)+orthogonal 16S RNA	Jinlai Zhang, Dongchang Qin	3226

Tianjin 2012 iGEM Team Parts Sandbox

1 W BBa_K821003 Coding 16S DNA sequence from rrnB Jinlai Zhang, Dongchang Qin 1894 2 W BBa_K821004 Regulatory PBad Promoter Jinlai Zhang, Dongchang Qin 1324 3 W BBa_K821005 Reporter CmR label from pKD3 Qinfeng Wu, Bin Jia 1014 4 W BBa_K821006 Project KanR label from pKD3 Jinlai Zhang, Dongchang Qin 1304 5 W BBa_K821007 Composite PL(promoter)+orthogonal 16S RNA Qinfeng Wu, Bin Jia 1957 6 W BBa_K821008 Composite PLtet+orthogonal 16S RNA Jinlai Zhang, Dongchang Qin 1956 7 W BBa_K821009 RBS Orthogonal RBS 1 Qinfeng Wu, Bin Jia 6 8 W BBa_K821010 Composite PLacI+Native RBS+RFP+Native RBS+GFP+Terminator Qinfeng Wu, Bin Jia 1833 9 W BBa_K821003 Composite PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator Jinlai Zhang, Dongchang Qin 1827

Agarose gel electrophoresis

7.14 t7 rbs' crtE

7.31 16s' RFP-GFP AMP

8.03 pSB1C3 K3 Digestion

8.16 16s' mutate to remove EcoRI

8.17 16S' pSB1C3

8.17 Mutate the RBS of Amp using PYES2

8.17 Yeast Assembly using pSB3C5R & pSB3K3

8.18 pBad-16S' remove EcorI, PSB1C3

9.2 6I 6G Gel extraction and verification

9.2 6I,6G P16S Gel extraction and verification

9.2 P16S digestion

9.16 yeast cPCR 1

9.16 yeast cPCR 2

81 16s GFP & RFP

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 <th style="background:#f680c4;">&nbsp;</th>
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 <td style="background:#fabfe1;">1</td>
-<td style="background:#fabfe1;">Working</td>
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 <td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821001">BBa_K821001</a></td>
 <td style="background:#fabfe1;">Reporter</td>
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 <td>2</td>
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 <td>Coding</td>
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 <td style="background:#fabfe1;">3</td>
-<td style="background:#fabfe1;">Working</td>
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 <td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821012">BBa_K821012</a></td>
 <td style="background:#fabfe1;">Generator</td>
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 <td style="background:#fabfe1;">1</td>
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 <td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
 <td style="background:#fabfe1;">Coding</td>
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 <td>2</td>
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 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821004">BBa_K821004</a></td>
 <td>Regulatory</td>
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 <td style="background:#fabfe1;">3</td>
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 <td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821005">BBa_K821005</a></td>
 <td style="background:#fabfe1;">Reporter</td>
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 <td>4</td>
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+<td>W</td>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821006">BBa_K821006</a></td>
 <td>Project</td>
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 <td style="background:#fabfe1;">5</td>
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 <td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821007">BBa_K821007</a></td>
 <td style="background:#fabfe1;">Composite</td>
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 <td>6</td>
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+<td>W</td>
 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821008">BBa_K821008</a></td>
 <td>Composite</td>
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 <td style="background:#fabfe1;">7</td>
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 <td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821009">BBa_K821009</a></td>
 <td style="background:#fabfe1;">RBS</td>
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 <td>8</td>
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 <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821010">BBa_K821010</a></td>
 <td>Composite</td>
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 <td style="background:#fabfe1;">9</td>
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 <td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
 <td style="background:#fabfe1;">Composite</td>

Team:Tianjin/Data