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Our parts :

Data about our favorite new parts :

1.Main Page: Dispersin generator for B. subtilis BBa_K802001 : This part associates the Bacillus subtilis Constitutive Promoter (P_veg) with the dispersin B gene (dspB). In our plasmid collection, this part is named pBK33 in the Chloramphenicol backbone and pBKH41 in the shuttle vector E. coli – B. subtilis. We worked with the plasmid pBKH41 for the tests and we tried two different genetic backgrounds : the NM522 strain to make test in E. coli and the 168 strain to make test in Bacillus subtilis.

2.Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low copy number plasmid in B. subtilis and a high copy number plasmid in E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. We determined antibiotic resistance thresholds and transformation efficiencies and compared to BBa_K802004


3.Main Page: Lysostaphin generator for B. subtilis BBa_K802000 : This part associates the Bacillus subtilis Constitutive Promoter (P_veg) with the lysostaphin gene. In our plasmid collection, this part is named pBK23 in the pSB1C3 backbone (Cm^R) and pBKL28 in the shuttle vector E. coli – B. subtilis. The pBKL28 plasmid was transformed in the NM522 strain to perform tests in E. coli and in the 168 strain to perform tests in Bacillus subtilis.


We've also characterized the following new parts :

Main Page: P_lac(B. subtilis)-RBS(E. coli)-GFP BBa_K802002 : This part is used to determine if the P_lac promoter works or not and especially how it works when it is activated by different concentration of the inducer IPTG. Within this part there is P_lac from Bacillus subtilis which is the promoter that induces production of XylR to form a positive biofilm. Then a RBS from E coli is added to finally enable production of GFP which is an easy measurable parameter using the amount of fluorescence.


Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high copy number plasmid in both B. subtilis and E. coli. It has a polylinker containing all 4 of the iGEM restriction sites.

Main Page: iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004 BBa_K802005 : This part is a custom designed polylinker which was cloned into the shuttle vectors BBa_K802004 and BBa_K802003 so that the plasmids would correspond to the iGEM format.

Main Page: BBa_K802009