Team:Tianjin/Data

From 2012.igem.org

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<tr>
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<td>1</td>
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<th style="background:#f680c4;">&nbsp;</th>
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<td>Working</td>
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<th style="background:#f680c4;">Name</th>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821001">BBa_K821001</a></td>
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<th style="background:#f680c4;">Type</th>
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<td>Reporter</td>
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<th style="background:#f680c4;">Description</th>
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<td>RFP Reporter with o-RBS</td>
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<th style="background:#f680c4;">Designer</th>
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<td>Qinfeng Wu, Bin Jia</td>
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<th style="background:#f680c4;">Length</th>
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<td>1059</td>
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<tr>
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<td style="background:#fabfe1;">1</td>
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<td style="background:#fabfe1;">Working</td>
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<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821001">BBa_K821001</a></td>
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<td style="background:#fabfe1;">Reporter</td>
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<td style="background:#fabfe1;">RFP Reporter with o-RBS</td>
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<td style="background:#fabfe1;">Qinfeng Wu, Bin Jia</td>
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<td style="background:#fabfe1;">1059</td>
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<tr>
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<td>3</td>
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<td style="background:#fabfe1;">3</td>
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<td>Working</td>
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<td style="background:#fabfe1;">Working</td>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821012">BBa_K821012</a></td>
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<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821012">BBa_K821012</a></td>
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<td>Generator</td>
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<td style="background:#fabfe1;">Generator</td>
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<td>PBad(promoter)+orthogonal 16S RNA</td>
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<td style="background:#fabfe1;">PBad(promoter)+orthogonal 16S RNA</td>
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<td>Jinlai Zhang, Dongchang Qin</td>
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<td style="background:#fabfe1;">Jinlai Zhang, Dongchang Qin</td>
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<td>3226</td>
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<td style="background:#fabfe1;">3226</td>
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<td>1</td>
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<td style="background:#fabfe1;">1</td>
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<td>Working</td>
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<td style="background:#fabfe1;">Working</td>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
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<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
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<td>Coding</td>
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<td style="background:#fabfe1;">Coding</td>
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<td>16S DNA sequence from rrnB</td>
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<td style="background:#fabfe1;">16S DNA sequence from rrnB</td>
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<td>Jinlai Zhang, Dongchang Qin</td>
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<td style="background:#fabfe1;">Jinlai Zhang, Dongchang Qin</td>
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<td>1894</td>
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<td style="background:#fabfe1;">1894</td>
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<td>3</td>
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<td style="background:#fabfe1;">3</td>
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<td>Working</td>
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<td style="background:#fabfe1;">Working</td>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821005">BBa_K821005</a></td>
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<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821005">BBa_K821005</a></td>
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<td>Reporter</td>
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<td style="background:#fabfe1;">Reporter</td>
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<td>CmR label from pKD3</td>
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<td style="background:#fabfe1;">CmR label from pKD3</td>
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<td>Qinfeng Wu, Bin Jia</td>
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<td style="background:#fabfe1;">Qinfeng Wu, Bin Jia</td>
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<td>1014</td>
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<td style="background:#fabfe1;">1014</td>
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<tr>
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<td>5</td>
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<td style="background:#fabfe1;">5</td>
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<td>Working</td>
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<td style="background:#fabfe1;">Working</td>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821007">BBa_K821007</a></td>
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<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821007">BBa_K821007</a></td>
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<td>Composite</td>
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<td style="background:#fabfe1;">Composite</td>
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<td>PL(promoter)+orthogonal 16S RNA</td>
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<td style="background:#fabfe1;">PL(promoter)+orthogonal 16S RNA</td>
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<td>Qinfeng Wu, Bin Jia</td>
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<td style="background:#fabfe1;">Qinfeng Wu, Bin Jia</td>
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<td>1957</td>
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<td style="background:#fabfe1;">1957</td>
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<td>7</td>
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<td style="background:#fabfe1;">7</td>
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<td>Working</td>
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<td style="background:#fabfe1;">Working</td>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821009">BBa_K821009</a></td>
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<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821009">BBa_K821009</a></td>
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<td>RBS</td>
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<td style="background:#fabfe1;">RBS</td>
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<td>Orthogonal RBS 1</td>
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<td style="background:#fabfe1;">Orthogonal RBS 1</td>
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<td>Qinfeng Wu, Bin Jia</td>
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<td style="background:#fabfe1;">Qinfeng Wu, Bin Jia</td>
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<td>6</td>
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<td style="background:#fabfe1;">6</td>
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<tr>
<tr>
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<td>9</td>
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<td style="background:#fabfe1;">9</td>
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<td>Working</td>
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<td style="background:#fabfe1;">Working</td>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
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<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
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<td>Composite</td>
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<td style="background:#fabfe1;">Composite</td>
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<td>PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator </td>
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<td style="background:#fabfe1;">PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator </td>
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<td>Jinlai Zhang, Dongchang Qin</td>
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<td style="background:#fabfe1;">Jinlai Zhang, Dongchang Qin</td>
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<td>1827</td>
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<td style="background:#fabfe1;">1827</td>
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Revision as of 15:38, 26 September 2012

Contents

BioBrick

Favorite Tianjin 2012 iGEM Team Parts

  Name Type Description Designer Length
1 Working BBa_K821001 Reporter RFP Reporter with o-RBS Qinfeng Wu, Bin Jia 1059
2 Working BBa_K821002 Coding Orthogonal 16S DNA sequence from rrnB Qinfeng Wu, Bin Jia 1894
3 Working BBa_K821012 Generator PBad(promoter)+orthogonal 16S RNA Jinlai Zhang, Dongchang Qin 3226

Tianjin 2012 iGEM Team Parts Sandbox

1 Working BBa_K821003 Coding 16S DNA sequence from rrnB Jinlai Zhang, Dongchang Qin 1894 2 Working BBa_K821004 Regulatory PBad Promoter Jinlai Zhang, Dongchang Qin 1324 3 Working BBa_K821005 Reporter CmR label from pKD3 Qinfeng Wu, Bin Jia 1014 4 Working BBa_K821006 Project KanR label from pKD3 Jinlai Zhang, Dongchang Qin 1304 5 Working BBa_K821007 Composite PL(promoter)+orthogonal 16S RNA Qinfeng Wu, Bin Jia 1957 6 Working BBa_K821008 Composite PLtet+orthogonal 16S RNA Jinlai Zhang, Dongchang Qin 1956 7 Working BBa_K821009 RBS Orthogonal RBS 1 Qinfeng Wu, Bin Jia 6 8 Working BBa_K821010 Composite PLacI+Native RBS+RFP+Native RBS+GFP+Terminator Qinfeng Wu, Bin Jia 1833 9 Working BBa_K821003 Composite PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator Jinlai Zhang, Dongchang Qin 1827

Agarose gel electrophoresis

7.14 t7 rbs' crtE
7.31 16s' RFP-GFP AMP


8.03 pSB1C3 K3 Digestion
8.16 16s' mutate to remove EcoRI


8.17 16S' pSB1C3
8.17 Mutate the RBS of Amp using PYES2


8.17 Yeast Assembly using pSB3C5R & pSB3K3
8.18 pBad-16S' remove EcorI, PSB1C3


9.2 6I 6G Gel extraction and verification
9.2 6I,6G P16S Gel extraction and verification


9.2 P16S digestion
9.16 yeast cPCR 1


9.16 yeast cPCR 2
81 16s GFP & RFP



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