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Revision as of 14:44, 26 September 2012 (view source) Beccaphilp (Talk | contribs) ← Older edit		Revision as of 14:54, 26 September 2012 (view source) Beccaphilp (Talk | contribs) Newer edit →
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	<b><p>BBa_K764033 Tetr promoter_rbs_ompA_BBa_K322921-Term</p></b>		<b><p>BBa_K764033 Tetr promoter_rbs_ompA_BBa_K322921-Term</p></b>
-		+	The complete construct of Newcastle iGEM 2010's biobrick BBa_K322921 for SacB. This was linked to the signal peptide for export from the cell and prevent damage to our E coli.
	<b><p>BBa_K764034 Pbad Weak promoter_rbs_ompA- BBa_K322921-Term</p></b>		<b><p>BBa_K764034 Pbad Weak promoter_rbs_ompA- BBa_K322921-Term</p></b>

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Revision as of 14:54, 26 September 2012

Due to the time available we were only able to get 14 of our potential Biobricks into pSB1C3 and sent into the registry. Over 30 more were waiting for there chance. Our project was certainly worth more than the sum of it's Registry parts!</font>

BBa_K764007 WbiP

Encodes the galactosytransferase wbiP that adds a β-D-Galactose donor sugar onto an α-D-GalNAc(N-Acetylgalactosamine) acceptor sugar to form a 1->3 glycosidic bond.

BBa_K764003 wfcA

Encodes the glucoronic acid transferase wfcA that adds a β-D-GlcA donor sugar onto an α-D-Mannose acceptor sugar to form a 1->2 glycosidic bond.

BBa_K764002 wbnK

Encodes the fucosyltransferase wbnK that adds a branched α-L-Fucose donor sugar onto a β-D-Galactose acceptor sugar to form a 1->2 glycosidic bond.

BBa_K764000 wbnJ

Encodes the galactosytransferase wbnJ that adds a β-D-Galactose donor sugar onto an α-D-GalNAc(N-Acetylgalactosamine) acceptor sugar to form a 1->3 glycosidic bond.

BBa_K764004 WclY</i>

Encodes the glycosyltransferase wclY that adds an α-D-GlcNAc (N-Acetylglucosamine) donor sugar onto a β-D-Galactose acceptor sugar to form a 1->4 glycosidic bond.

BBa_K764033 Tetr promoter_rbs_ompA_BBa_K322921-Term

The complete construct of Newcastle iGEM 2010's biobrick BBa_K322921 for SacB. This was linked to the signal peptide for export from the cell and prevent damage to our E coli.

BBa_K764034 Pbad Weak promoter_rbs_ompA- BBa_K322921-Term

BBa_K764026 Cyclodextrin glycosyltransferase + BBa_B0014

BBa_K764025 Hyaluronan synthase + BBa_B0014

The enzyme hyaluronan synthase is responsible for generating hyaluronan from D-glucuronic acid and D-N-acetylglucosamine, linked via alternating β-1,4 and β-1,3 glycosidic bonds.

BBa_K764024 BBa_K322921 + BBa_B0014

BBa_K764022 Hyaluronan synthase

Encodes hyaluronan synthase (hasA) that performs the following two reactions:

UDP-α-N-acetyl-D-glucosamine + β-D-glucuronosyl-(1->3)-N-acetyl-β-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-β-D-glucosaminyl-(1->4)-β-D-glucuronosyl-(1->3)-N-acetyl-β-D-glucosaminyl-(1->4)-(nascent hyaluronan)

UDP-α-D-glucuronate + N-acetyl-β-D-glucosaminyl-(1->4)-β-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + β-D-glucuronosyl-(1->3)-N-acetyl-β-D-glucosaminyl-(1->4)-β-D-glucuronosyl-(1->3)-(nascent hyaluronan).

This generates hyaluronan, a virulence factor in streptococcus pyogenes if linked to lipid A molecules in the cell wall. Hyaluronan is also present in human joints and skin as a lubricant.

BBa_K764023 Cyclodextrin glycosyltransferase

Performs the enzymatic conversion of starch into variable sized cyclic oligosaccharides. This glycosyltransferase (cgt) from Bacillis sp 1011 produces alpha-cyclodextrin, a six membered ring of α-D-glucopyranoside units linked 1->4 glycosidic bonds.

BBa_K764009 wclY + BBa_B0014

BBa_K764011 wclY + BBa_B0014