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Team:SDU-Denmark/geneseq
From 2012.igem.org
| Line 260: | Line 260: | ||
3' GACGTC </br></br> | 3' GACGTC </br></br> | ||
</p> | </p> | ||
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<h2>Our plant genes:</h2> | <h2>Our plant genes:</h2> | ||
<p> | <p> | ||
First primer Extended Start codon Stop codon Restriction site Mutagenesis primer | First primer Extended Start codon Stop codon Restriction site Mutagenesis primer | ||
| - | 1-FFT: | + | <b>1-FFT:</b> |
| - | + | AGGAGGTGTCACCATGCAAACCCCTGAACCCTTTACAGACCTTGAACATGAACCCCACACACCCCTACTGGACCACCACCACAACCCACCACCACAA</br> | |
| + | ACCACCACAAAACCTTTGTTCACCAGGGTTGTGTCCGGTGTCACCTTTGTTTTATTCTTCTTTGGTTTCGCTATCGTATTCATTGTTCTCAACCAAC</br> | ||
| + | AGAACTCTTCTGTTCGTATCGTCACCAATTCGGAGAAATCTTTTATAAGGTATTCGCAGACCGATCGCTTGTCGTGGGAACGGACCGCTTTTCATTT</br> | ||
| + | TCAGCCTGCCAAGAATTTTATTTACGATCCAGATGGTCAGTTGTTTCACATGGGCTGGTACCATATGTTCTATCAATACAACCCATACGCACCGGTT</br> | ||
| + | TGGGGCAATATGTCATGGGGTCACTCAGTGTCCAAAGACATGATCAACTGGTACGAGCTGCCAGTCGCTATGGTCCCGACCGAATGGTATGATATCG</br> | ||
| + | AGGGCGTCTTATCCGGGTCTACCACGGTCCTTCCAAACGGTCAGATCTTTGCATTGTATACTGGGAACGCTAATGATTTTTCCCAATTACAATGCAA</br> | ||
| + | AGCTGTACCCGTAAACTTATCTGACCCGCTTCTTATTGAGTGGGTCAAGTATGAGGATAACCCAATCCTGTACACTCCACCAGGGATTGGGTTAAAG</br> | ||
| + | GACTATCGGGACCCGTCAACAGTCTGGACAGGTCCCGATGGAAAGCATAGGATGATCATGGGAACTAAACGTGGCAATACAGGCATGGTACTTGTTT</br> | ||
| + | ACTATACCACTGATTACACGAACTACGAGTTGTTGGATGAGCCGTTGCACTCTGTTCCCAACACCGATATGTGGGAATGCGTCGACTTTTACCCGGT</br> | ||
| + | TTCGTTAACCAATGATAGTGCACTTGATATGGCGGCCTATGGGTCGGGTATCAAACACGTTATTAAAGAAAGTTGGGAGGGACATGGAATGGATTGG</br> | ||
| + | TATTCAATCGGGACATATGACGCGATAAATGATAAATGGACTCCCGATAACCCGGAACTAGATGTCGGTATCGGGTTACGGTGCGATTACGGGAGGT</br> | ||
| + | TTTTTGCATCAAAGAGTCTTTATGACCCATTGAAGAAAAGGAGGATCACTTGGGGTTATGTTGGAGAATCAGATAGTGCTGATCAGGACCTCTCAAG</br> | ||
| + | AGGATGGGCTACTGTTTATAATGTTGGAAGAACAATTGTACTAGATAGAAAGACCGGGACCCATTTACTTCATTGGCCCGTTGAGGAAGTCGAGAGT</br> | ||
| + | TTGAGATACAACGGTCAGGAGTTTAAAGAGATCAAGCTAGAGCCCGGTTCAATCATTCCACTCGACATAGGCACGGCTACACAGTTGGACATAGTTG</br> | ||
| + | CAACATTTGAGGTGGATCAAGCAGCGTTGAACGCGACAAGTGAAACCGATGATATTTATGGTTGCACCACTAGCTTAGGTGCAGCCCAAAGGGGAAG</br> | ||
| + | TTTGGGACCATTTGGTCTTGCGGTTCTAGCCGATGGAACCCTTTCTGAGTTAACTCCGGTTTATTTCTATATAGCTAAAAAGGCAGATGGAGGTGTG</br> | ||
| + | TCGACACATTTTTGTACCGATAAGCTAAGGTCATCACTAGATTATGATGGGGAGAGAGTGGTGTATGGGGGCACTGTTCCTGTGTTAGATGATGAAG</br> | ||
| + | AACTCACAATGAGGCTATTGGTGGATCATTCGATAGTGGAGGGGTTTGCGCAAGGAGGAAGGACGGTTATAACATCAAGGGCGTATCCAACAAAAGC</br> | ||
| + | GATATACGAACAAGCGAAGCTGTTCTTGTTCAACAACGCCACAGGTACGAGTGTGAAAGCATCTCTCAAGATTTGGCAAATGGCTTCTGCACCAATT</br> | ||
| + | CATCAATACCCTTTTTAATTACCGGCTATCGCTATCCTTTTTGTTATTG</br> | ||
Revision as of 14:52, 26 September 2012
Standard restriction sites:
EcoRI: 5' GAATTC 3' CTTAAG XbaI: 5' T*CTAGA 3' AGATC*T SpeI: 5' A*CTAGT 3' TGATC*A PstI: 5' CTGCAG 3' GACGTC
Our plant genes:
First primer Extended Start codon Stop codon Restriction site Mutagenesis primer 1-FFT: AGGAGGTGTCACCATGCAAACCCCTGAACCCTTTACAGACCTTGAACATGAACCCCACACACCCCTACTGGACCACCACCACAACCCACCACCACAA ACCACCACAAAACCTTTGTTCACCAGGGTTGTGTCCGGTGTCACCTTTGTTTTATTCTTCTTTGGTTTCGCTATCGTATTCATTGTTCTCAACCAAC AGAACTCTTCTGTTCGTATCGTCACCAATTCGGAGAAATCTTTTATAAGGTATTCGCAGACCGATCGCTTGTCGTGGGAACGGACCGCTTTTCATTT TCAGCCTGCCAAGAATTTTATTTACGATCCAGATGGTCAGTTGTTTCACATGGGCTGGTACCATATGTTCTATCAATACAACCCATACGCACCGGTT TGGGGCAATATGTCATGGGGTCACTCAGTGTCCAAAGACATGATCAACTGGTACGAGCTGCCAGTCGCTATGGTCCCGACCGAATGGTATGATATCG AGGGCGTCTTATCCGGGTCTACCACGGTCCTTCCAAACGGTCAGATCTTTGCATTGTATACTGGGAACGCTAATGATTTTTCCCAATTACAATGCAA AGCTGTACCCGTAAACTTATCTGACCCGCTTCTTATTGAGTGGGTCAAGTATGAGGATAACCCAATCCTGTACACTCCACCAGGGATTGGGTTAAAG GACTATCGGGACCCGTCAACAGTCTGGACAGGTCCCGATGGAAAGCATAGGATGATCATGGGAACTAAACGTGGCAATACAGGCATGGTACTTGTTT ACTATACCACTGATTACACGAACTACGAGTTGTTGGATGAGCCGTTGCACTCTGTTCCCAACACCGATATGTGGGAATGCGTCGACTTTTACCCGGT TTCGTTAACCAATGATAGTGCACTTGATATGGCGGCCTATGGGTCGGGTATCAAACACGTTATTAAAGAAAGTTGGGAGGGACATGGAATGGATTGG TATTCAATCGGGACATATGACGCGATAAATGATAAATGGACTCCCGATAACCCGGAACTAGATGTCGGTATCGGGTTACGGTGCGATTACGGGAGGT TTTTTGCATCAAAGAGTCTTTATGACCCATTGAAGAAAAGGAGGATCACTTGGGGTTATGTTGGAGAATCAGATAGTGCTGATCAGGACCTCTCAAG AGGATGGGCTACTGTTTATAATGTTGGAAGAACAATTGTACTAGATAGAAAGACCGGGACCCATTTACTTCATTGGCCCGTTGAGGAAGTCGAGAGT TTGAGATACAACGGTCAGGAGTTTAAAGAGATCAAGCTAGAGCCCGGTTCAATCATTCCACTCGACATAGGCACGGCTACACAGTTGGACATAGTTG CAACATTTGAGGTGGATCAAGCAGCGTTGAACGCGACAAGTGAAACCGATGATATTTATGGTTGCACCACTAGCTTAGGTGCAGCCCAAAGGGGAAG TTTGGGACCATTTGGTCTTGCGGTTCTAGCCGATGGAACCCTTTCTGAGTTAACTCCGGTTTATTTCTATATAGCTAAAAAGGCAGATGGAGGTGTG TCGACACATTTTTGTACCGATAAGCTAAGGTCATCACTAGATTATGATGGGGAGAGAGTGGTGTATGGGGGCACTGTTCCTGTGTTAGATGATGAAG AACTCACAATGAGGCTATTGGTGGATCATTCGATAGTGGAGGGGTTTGCGCAAGGAGGAAGGACGGTTATAACATCAAGGGCGTATCCAACAAAAGC GATATACGAACAAGCGAAGCTGTTCTTGTTCAACAACGCCACAGGTACGAGTGTGAAAGCATCTCTCAAGATTTGGCAAATGGCTTCTGCACCAATT CATCAATACCCTTTTTAATTACCGGCTATCGCTATCCTTTTTGTTATTG
