Team:Exeter/Results/biobricks

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<b><p>BBa_K764007 <i>WbiP</i></p></b>
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<p>Encodes the galactosytransferase <i>wbiP</i> that adds a β-D-Galactose donor sugar onto an α-D-GalNAc(N-Acetylgalactosamine) acceptor sugar to form a 1->3 glycosidic bond. </p>
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<b><p>BBa_K764003 <i>wfcA</i></p></b>
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<p>Encodes the glucoronic acid transferase <i>wfcA</i> that adds a β-D-GlcA donor sugar onto an α-D-Mannose acceptor sugar to form a 1->2 glycosidic bond. </p>
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<b><p>BBa_K764002 <i>wbnK</i></p></b>
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<p>Encodes the fucosyltransferase <i>wbnK</i> that adds a branched α-L-Fucose donor sugar onto a β-D-Galactose acceptor sugar to form a 1->2 glycosidic bond. </p>
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<b><p>BBa_K764000 <i>wbnJ</i></p></b>
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<p>Encodes the galactosytransferase <i>wbnJ</i> that adds a β-D-Galactose donor sugar onto an α-D-GalNAc(N-Acetylgalactosamine) acceptor sugar to form a 1->3 glycosidic bond. </p>
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<b><p>BBa_K764004 WclY</i></p></b>
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<p>Encodes the glycosyltransferase <i>wclY</i> that adds an α-D-GlcNAc (N-Acetylglucosamine) donor sugar onto a β-D-Galactose acceptor sugar to form a 1->4 glycosidic bond. </p>
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<b><p>BBa_K764033 Tetr promoter_rbs_ompA_BBa_K322921-Term</p></b>
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<b><p>BBa_K764034 Pbad Weak promoter_rbs_ompA- BBa_K322921-Term</p></b>
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<b><p>BBa_K764026 Cyclodextrin glycosyltransferase + BBa_B0014</p></b>
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<b><p>BBa_K764025 Hyaluronan synthase + BBa_B0014 </p></b>
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<b><p>BBa_K764024 BBa_K322921 + BBa_B0014 </p></b>
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<b><p>BBa_K764022 Hyaluronan synthase </p></b>
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<p>Encodes hyaluronan synthase (hasA) that performs the following two reactions: </p>
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<p>UDP-α-N-acetyl-D-glucosamine + β-D-glucuronosyl-(1->3)-N-acetyl-β-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-β-D-glucosaminyl-(1->4)-β-D-glucuronosyl-(1->3)-N-acetyl-β-D-glucosaminyl-(1->4)-(nascent hyaluronan) </p>
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<p>UDP-α-D-glucuronate + N-acetyl-β-D-glucosaminyl-(1->4)-β-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + β-D-glucuronosyl-(1->3)-N-acetyl-β-D-glucosaminyl-(1->4)-β-D-glucuronosyl-(1->3)-(nascent hyaluronan). </p>
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<b><p>BBa_K764023 Cyclodextrin glycosyltransferase </p></b>
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<p>Encodes the cyclodextrin glycosyltransferase cgt that cyclises part of a (1->4)-α-D-glucan chain by formation of a (1->4)-α-D-glucosidic bond. </p>
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<b><p>BBa_K764009 <i>wclY</i> + BBa_B0014 </p></b>
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<b><p>BBa_K764011 <i>wclY</i> + BBa_B0014</p></b>

Revision as of 14:25, 26 September 2012


BBa_K764007 WbiP

Encodes the galactosytransferase wbiP that adds a β-D-Galactose donor sugar onto an α-D-GalNAc(N-Acetylgalactosamine) acceptor sugar to form a 1->3 glycosidic bond.


BBa_K764003 wfcA

Encodes the glucoronic acid transferase wfcA that adds a β-D-GlcA donor sugar onto an α-D-Mannose acceptor sugar to form a 1->2 glycosidic bond.

BBa_K764002 wbnK

Encodes the fucosyltransferase wbnK that adds a branched α-L-Fucose donor sugar onto a β-D-Galactose acceptor sugar to form a 1->2 glycosidic bond.

BBa_K764000 wbnJ

Encodes the galactosytransferase wbnJ that adds a β-D-Galactose donor sugar onto an α-D-GalNAc(N-Acetylgalactosamine) acceptor sugar to form a 1->3 glycosidic bond.

BBa_K764004 WclY</i>

Encodes the glycosyltransferase wclY that adds an α-D-GlcNAc (N-Acetylglucosamine) donor sugar onto a β-D-Galactose acceptor sugar to form a 1->4 glycosidic bond.

BBa_K764033 Tetr promoter_rbs_ompA_BBa_K322921-Term


BBa_K764034 Pbad Weak promoter_rbs_ompA- BBa_K322921-Term


BBa_K764026 Cyclodextrin glycosyltransferase + BBa_B0014


BBa_K764025 Hyaluronan synthase + BBa_B0014


BBa_K764024 BBa_K322921 + BBa_B0014


BBa_K764022 Hyaluronan synthase

Encodes hyaluronan synthase (hasA) that performs the following two reactions:

UDP-α-N-acetyl-D-glucosamine + β-D-glucuronosyl-(1->3)-N-acetyl-β-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-β-D-glucosaminyl-(1->4)-β-D-glucuronosyl-(1->3)-N-acetyl-β-D-glucosaminyl-(1->4)-(nascent hyaluronan)

UDP-α-D-glucuronate + N-acetyl-β-D-glucosaminyl-(1->4)-β-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + β-D-glucuronosyl-(1->3)-N-acetyl-β-D-glucosaminyl-(1->4)-β-D-glucuronosyl-(1->3)-(nascent hyaluronan).


BBa_K764023 Cyclodextrin glycosyltransferase

Encodes the cyclodextrin glycosyltransferase cgt that cyclises part of a (1->4)-α-D-glucan chain by formation of a (1->4)-α-D-glucosidic bond.


BBa_K764009 wclY + BBa_B0014


BBa_K764011 wclY + BBa_B0014
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