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Team:LMU-Munich/Data/Inverter
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[[File:LMU Inverter graph.png|600px]] | [[File:LMU Inverter graph.png|600px]] | ||
| - | The Inverter is composed of the pBAD promoter (lacking the repressor binding sites) which produces the short RNA RyhB. RyhB binds to uof and masks the Shine Dalgarno sequence, | + | The Inverter is composed of the pBAD promoter (lacking the repressor binding sites), which produces the short RNA RyhB. RyhB binds to uof<sub>CGU</sub> and masks the Shine Dalgarno sequence, thereby translationally repressing it. Uof<sub>CGU</sub> is translationally fused to ''lacZalpha'', and therefore also translationally repressed. Uof<sub>CGU</sub>-''lacZalpha'' is produced by an [http://partsregistry.org/Part:BBa_R0011 IPTG-inducible Promoter] which is induced with 1mM IPTG. The P<sub>''BAD''</sub> is titrated with Arabinose. The higher the arabinose concentration is, the higher the RyhB concentration and consequently the ''lacZalpha'' translational repression. |
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Revision as of 12:29, 26 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
ONPG assay of the Inverter with lacZalpha
The Inverter is composed of the pBAD promoter (lacking the repressor binding sites), which produces the short RNA RyhB. RyhB binds to uofCGU and masks the Shine Dalgarno sequence, thereby translationally repressing it. UofCGU is translationally fused to lacZalpha, and therefore also translationally repressed. UofCGU-lacZalpha is produced by an [http://partsregistry.org/Part:BBa_R0011 IPTG-inducible Promoter] which is induced with 1mM IPTG. The PBAD is titrated with Arabinose. The higher the arabinose concentration is, the higher the RyhB concentration and consequently the lacZalpha translational repression.
