Team:Korea U Seoul/Human Practice/Ownership Innovation

     The previous attempts purposed to prevent plant disease have been conducted by genetically manipulating plants known as GMO(Genetically Modified Organism). However this approach has side effects. For example, GMO has different protein composition from foods that we usually take, so it could cause unknown effects to human body. Also, these modified products could cause allergy. Like this, the stability of GMO is not substantiated yet. On the other hand, our approach to prevent plant disease was conducted by modifying E. coli . As the modified E. coli kills the problematic bacteria and suicides, there is no residue and so the side effects are fewer than the previous approach. Aside from this, the way we designed is alternative method for agricultural pesticides. As you know, agricultural pesticides are bad for health and also environment.

     Therehave been many researches on logic circuits in biological system, especially inthe transcriptional system. A set of genetic logic gates (AND, OR and XOR) havebeen constructed and verified experimentally. We used each genetic logic gateas building blocks for creating transcriptional binary full adder. Unlikeprevious studies, our operation contains successive logic gates, so we neededto connect each logic gate. To address this issue, we employed quorum sensingsystem in various microorganisms. Quorum sensing is realized by a variety ofsignal molecules including isopropylthiogalactoside (IPTG), arabinose andN-(3-Oxohexanoyl)-L-homoserine lactone (AHL). Among these, we selected XX as asignal molecule in the whole transcriptional full adder system forsimplification. Besides experimental design, we constructed computational modelwith R which is one of statistical programs. By implementing Hill equation and biologicaldiffusion dynamics as a theoretical base, we obtained curves showing concentrationsof the signal molecules.