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Team:Bielefeld-Germany/Outlook
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This page will soon report about annoying substrates in our water that we would like to deal with during our project. | This page will soon report about annoying substrates in our water that we would like to deal with during our project. | ||
| + | == Shuttle vector == | ||
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| + | The [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#11 shuttle vector (pECPP11JS)] could be constructed. The following step is to do site directed mutagenesis to eliminate the ilegale XbaI restriction site in ''his4'' gene. At next the genotype of the ''P. pastoris'' mutants have to be characteriazed as Mut<sup>+</sup> or Mut<sup>s</sup> transformants via PCR with 5AOX-Genotype-FW and TT-Genotype-RV primer. Afterwards the GFP::pECPP11JS construct have to sequenced and transformed into ''P. pastoris'' cells. The same procedure have to be repeated with the laccases from '' Trametes versiculor'' TVEL5 and from ''Pycnoporus cinnabarinus'' PCIL35. | ||
Revision as of 11:01, 26 September 2012
Outlook
This page will soon report about annoying substrates in our water that we would like to deal with during our project.
Shuttle vector
The shuttle vector (pECPP11JS) could be constructed. The following step is to do site directed mutagenesis to eliminate the ilegale XbaI restriction site in his4 gene. At next the genotype of the P. pastoris mutants have to be characteriazed as Mut+ or Muts transformants via PCR with 5AOX-Genotype-FW and TT-Genotype-RV primer. Afterwards the GFP::pECPP11JS construct have to sequenced and transformed into P. pastoris cells. The same procedure have to be repeated with the laccases from Trametes versiculor TVEL5 and from Pycnoporus cinnabarinus PCIL35.
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