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Team:Grenoble/Biology/Notebook/July/week 33
From 2012.igem.org
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<h1>August</h1> | <h1>August</h1> | ||
| - | a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/August">Week 31</a> • | + | <a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/August">Week 31</a> • |
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_32">Week 32</a> • | <a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_32">Week 32</a> • | ||
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_33">Week 33</a> • | <a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_33">Week 33</a> • | ||
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competent cells (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Competence">protocol</a>) with pSB3C5.<br/> | competent cells (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Competence">protocol</a>) with pSB3C5.<br/> | ||
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{{:Team:Grenoble/script}} | {{:Team:Grenoble/script}} | ||
{{:Team:Grenoble/menu}} | {{:Team:Grenoble/menu}} | ||
Revision as of 10:51, 26 September 2012
August
Week 31 • Week 32 • Week 33 • Week 34 • Week 35Week 33: August 13th to 19th
Goal of the week:
Assembly of:- pAra/Bad_RBS_GFP and RBS_Cya.
- pompC and mcherry
- Tap and EnvZ
Monday, August 13th:
We did some verification PCRs on miniprep with HF Phusion enzyme (protocol) in order to check the Gibson Assembly products (12/09/09>.Annealing temperature = 55°C.
To separate (protocol) the PCR products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.
It showed no significant results (data not shown).
Tuesday, August 14th:
We did an experiment (protocol) in order to test the "AND" gate (CRP-cAMP & araC) without the amplifier module.We transformed (new protocol) BW25113 WT competent cells (protocol) with pSB3C5.
