Team:KAIT Japan/Notebook

From 2012.igem.org

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::PCR Product
::PCR Product
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:<big>'''Date:8/13'''</big>
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:<big>'''Date:8/13'''</big> Confirmed of electrophoresis by PCR product and Ligation of the TA vector
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::Confirmed of electrophoresis by PCR product and Ligation of the TA vector
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:<big>'''Date:8/14'''</big>
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:<big>'''Date:8/14'''</big> Transformation
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::Transformation
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:<big>'''Date:8/25'''</big>
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:<big>'''Date:8/25'''</big> Miniprep
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::Miniprep
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:<big>'''Date:9/10'''</big>
:<big>'''Date:9/10'''</big>
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::
 
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:<big>'''Date:9/11'''</big>
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:<big>'''Date:9/11'''</big> Primer design
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::Primer design
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:<big>'''Date:9/12'''</big>
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:<big>'''Date:9/12'''</big> Miniprep,Ligation
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::Miniprep,Ligation
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:<big>'''Date:9/13'''</big>
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:<big>'''Date:9/13'''</big> Electrophoresis,Refinement of DNA
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::Electrophoresis,Refinement of DNA
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:<big>'''Date:9/14'''</big>
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:<big>'''Date:9/14'''</big> Colony PCR
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::Colony PCR
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:<big>'''Date:9/15'''</big>
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:<big>'''Date:9/15'''</big> Ligation
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::Ligation
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:<big>'''Date:9/16'''</big>
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:<big>'''Date:9/16'''</big> PCR,Ligation
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::PCR,Ligation
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:<big>'''Date:9/17'''</big>
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:<big>'''Date:9/17'''</big> PCR,Ligation
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::PCR,Ligation
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:<big>'''Date:9/19'''</big>
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:<big>'''Date:9/19'''</big> Ligation
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::Ligation
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:<big>'''Date:9/20'''</big>
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:<big>'''Date:9/20'''</big> Enzyme inactivation,Ligation
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::Enzyme inactivation,Ligation
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:<big>'''Date:9/22'''</big>
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:<big>'''Date:9/22'''</big> Transformation,Colony PCR,Electrophoresis,Miniprep
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::Transformation,Colony PCR,Electrophoresis,Miniprep
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:<big>'''Date:9/23'''</big> Colony PCR,Electrophoresis,Streak
:<big>'''Date:9/23'''</big> Colony PCR,Electrophoresis,Streak

Revision as of 10:29, 26 September 2012

KAIT Japan2012 logo.png
KAIT Japan2012 Sub.png
Kaitjapan iGEM official.logo.png


Kaitjapan.home.png

Home

Kaitjapan project.png

Project

Kaitjapan parts.png

Parts

Kaitjapan protocol.png

Protocol

Kaitjapan notebook.png

Notebook

Kaitjapan results.png

Results

Kaitjapan safety.png

Safety

Kaitjapan human practice.png

Human Practice

Kaitjapan team.png

Team

Notebook

Our team pushed forward an experiment while making trial and error every day.

Creating parts of Tar methylation region

Date:8/9
Colony PCR
→Reflection:We Took E.coli too many.You should take less E.coli.
→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles)
Date:8/11
The purified DNA
→Reflection:Band was less.
PCR Product
Date:8/13 Confirmed of electrophoresis by PCR product and Ligation of the TA vector
Date:8/14 Transformation
Date:8/25 Miniprep
Date:9/10
Date:9/11 Primer design
Date:9/12 Miniprep,Ligation
Date:9/13 Electrophoresis,Refinement of DNA
Date:9/14 Colony PCR
Date:9/15 Ligation
Date:9/16 PCR,Ligation
Date:9/17 PCR,Ligation
Date:9/19 Ligation
Date:9/20 Enzyme inactivation,Ligation
Date:9/22 Transformation,Colony PCR,Electrophoresis,Miniprep
Date:9/23 Colony PCR,Electrophoresis,Streak
Date:9/25 Colony PCR

Creating parts of azurin

Date:8/16
Colony PCR
Ligation
Date:8/18
Colony PCR
Date:8/20
DNA extraction and purification of P.aeruginosa
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