Team:Colombia/Notebook/Journal

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(Chitinase)
(Chitinase)
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== Chitinase  ==
== Chitinase  ==
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-'' Trichoderma spp.''  
-'' Trichoderma spp.''  
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For each one we obtained accesion numbers:
For each one we obtained accesion numbers:
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-'' Trichoderma spp.''    : [http://www.ncbi.nlm.nih.gov/nucest/BM077089.2 BM077089.2]
-'' Trichoderma spp.''    : [http://www.ncbi.nlm.nih.gov/nucest/BM077089.2 BM077089.2]
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Each one was tested using codon usage for bacteria (transl_table=11, [www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi NCBI link]) to determine if the chitinase could be used. '''All of the sequences were suitable for use'''.
Each one was tested using codon usage for bacteria (transl_table=11, [www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi NCBI link]) to determine if the chitinase could be used. '''All of the sequences were suitable for use'''.
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We selected ''Colletotrichum spp.'' and ''Trichoderma spp.'' sequences to design primers.
We selected ''Colletotrichum spp.'' and ''Trichoderma spp.'' sequences to design primers.
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'''Primers'''
'''Primers'''

Revision as of 20:53, 26 June 2012

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Chitinase

To determine which chitinase is going to be used, we screened chitinase from 3 different species since the chitinase of different Vibrio spp. was not suitable or the strain was found (Alivibrio fischeri ES114, Alivibrio fischeri M11):

- Arabidopsis thaliana

- Colletotrichum spp.

- Trichoderma spp.


For each one we obtained accesion numbers:

- Arabidopsis thaliana: [http://www.ncbi.nlm.nih.gov/nuccore/AY099810.2 AY099810.2]

- Colletotrichum spp. : [http://www.ncbi.nlm.nih.gov/nucest/GW342409.1 GW342409.1]

- Trichoderma spp.  : [http://www.ncbi.nlm.nih.gov/nucest/BM077089.2 BM077089.2]



Each one was tested using codon usage for bacteria (transl_table=11, [www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi NCBI link]) to determine if the chitinase could be used. All of the sequences were suitable for use.


We selected Colletotrichum spp. and Trichoderma spp. sequences to design primers.


Primers

- Colletotrichum spp. : - Trichoderma spp.  :