Team:WashU/Protocols/LBPlates

From 2012.igem.org

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#Autoclave mixture for 20 minutes on liquid cycle.
#Autoclave mixture for 20 minutes on liquid cycle.
#Allow mixture to cool to around 55 degrees Celsius.
#Allow mixture to cool to around 55 degrees Celsius.
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+
#(optional). add appropriate amount of antibiotic. Final concentrations are given. Ampicillin-100ug/ml, Kanamycin-50ug/ml, Tetracycline-15ug/ml, Chloramphenicol-100ug/ml
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(optional). add appropriate amount of antibiotic. Final concentrations are given. Ampicillin-100ug/ml, Kanamycin-50ug/ml, Tetracycline-15ug/ml, Chloramphenicol-100ug/ml
+
-
 
+
#Pour 25 ml of agar mixture into plates, and allow to solidify.
#Pour 25 ml of agar mixture into plates, and allow to solidify.
#Use, or store in refrigerator.
#Use, or store in refrigerator.

Revision as of 19:40, 26 June 2012


LB plate

Making the plate

  1. Mix 25 grams of LB broth powder and 10 grams of agar with 1 Liter of deionized water.
  2. Shake the container until all of the agar and broth powder dissolves.
  3. Autoclave mixture for 20 minutes on liquid cycle.
  4. Allow mixture to cool to around 55 degrees Celsius.
  5. (optional). add appropriate amount of antibiotic. Final concentrations are given. Ampicillin-100ug/ml, Kanamycin-50ug/ml, Tetracycline-15ug/ml, Chloramphenicol-100ug/ml
  6. Pour 25 ml of agar mixture into plates, and allow to solidify.
  7. Use, or store in refrigerator.
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