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Team:Hong Kong-CUHK/DOC NBK SEPT
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<p class="aloveofthunder" style="line-height:normal; margin-bottom:35px">NOTEBOOK - SEPT</p> | <p class="aloveofthunder" style="line-height:normal; margin-bottom:35px">NOTEBOOK - SEPT</p> | ||
<ol> | <ol> | ||
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<li>Run gel of product from enzyme restriction cut (HsSRI-HsHtrI-Ectar gene) and the size of the bandings are correct, followed by gel purification. </li> | <li>Run gel of product from enzyme restriction cut (HsSRI-HsHtrI-Ectar gene) and the size of the bandings are correct, followed by gel purification. </li> | ||
<li>Ligation is done and the product is being transformed to Top10 competent cells, followed by spread plate. </li> | <li>Ligation is done and the product is being transformed to Top10 competent cells, followed by spread plate. </li> | ||
<li>Pick clones from the plate containing cells that contain NpSRII-NpHtrII-Ectsr gene. </li> | <li>Pick clones from the plate containing cells that contain NpSRII-NpHtrII-Ectsr gene. </li> | ||
<li>Bacterial cultures of picked clones that contain NpSRII-NpHtrII-Ectsr gene are being sent for sequencing. </li> | <li>Bacterial cultures of picked clones that contain NpSRII-NpHtrII-Ectsr gene are being sent for sequencing. </li> | ||
| - | + | </br> | |
<li>Testing cells that contain NpSRII-NpHtrII-Ectsr gene under monochromic light using 0.5% soft agar plate while another identical plate is being place in the dark for comparison. </li> | <li>Testing cells that contain NpSRII-NpHtrII-Ectsr gene under monochromic light using 0.5% soft agar plate while another identical plate is being place in the dark for comparison. </li> | ||
<li>Pick clones from the plate containing cells that contain NpSRII-NpHtrII-Ectar gene. </li> | <li>Pick clones from the plate containing cells that contain NpSRII-NpHtrII-Ectar gene. </li> | ||
<li>Bacterial cultures of picked clones that contain NpSRII-NpHtrII-Ectar gene are being sent for sequencing. </li> | <li>Bacterial cultures of picked clones that contain NpSRII-NpHtrII-Ectar gene are being sent for sequencing. </li> | ||
| - | + | </br> | |
<li>Testing cells that contain NpSRII-NpHtrII-Ectar gene under monochromic light using 0.5% soft agar plate while another identical plate is being place in the dark for comparison. </li> | <li>Testing cells that contain NpSRII-NpHtrII-Ectar gene under monochromic light using 0.5% soft agar plate while another identical plate is being place in the dark for comparison. </li> | ||
<li>Pick clones from the plate containing cells that contain HsSRI-HsHtrI-Ectar gene. </li> | <li>Pick clones from the plate containing cells that contain HsSRI-HsHtrI-Ectar gene. </li> | ||
<li>Bacterial cultures of picked clones that contain HsSRI-HsHtrI-Ectar gene are being sent for sequencing. </li> | <li>Bacterial cultures of picked clones that contain HsSRI-HsHtrI-Ectar gene are being sent for sequencing. </li> | ||
| - | + | </br> | |
<li>Testing cells that contain HsSRI-HsHtrI-Ectar gene under monochromic light using 0.5% soft agar plate while another identical plate is being place in the dark for comparison. </li> | <li>Testing cells that contain HsSRI-HsHtrI-Ectar gene under monochromic light using 0.5% soft agar plate while another identical plate is being place in the dark for comparison. </li> | ||
<li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectsr-standard backbone gene are correct. A new biobrick is successfully made. </li> | <li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectsr-standard backbone gene are correct. A new biobrick is successfully made. </li> | ||
<li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-standard backbone gene are correct. A new biobrick is successfully made. </li> | <li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-standard backbone gene are correct. A new biobrick is successfully made. </li> | ||
| - | + | </br> | |
<li>Sequencing results shown that the sequence of plasmids containing HsSRI-HsHtrI-Ectar-standard backbone gene are correct. A new biobrick is successfully made. </li> | <li>Sequencing results shown that the sequence of plasmids containing HsSRI-HsHtrI-Ectar-standard backbone gene are correct. A new biobrick is successfully made. </li> | ||
| - | <li>Characterization of promoter Bba_J23100 using | + | <li>Characterization of promoter Bba_J23100 using DH5α. </li> |
| - | <li>Characterization of promoter Bba_J23100 using | + | <li>Characterization of promoter Bba_J23100 using TOP10. </li> |
<li>Characterization of promoter Bba_J23100 using BL21. </li> | <li>Characterization of promoter Bba_J23100 using BL21. </li> | ||
| - | + | </br> | |
| - | <li>Repeat characterization of promoter Bba_J23100 using | + | <li>Repeat characterization of promoter Bba_J23100 using DH5α. </li> |
| - | <li>Repeat characterization of promoter Bba_J23100 using | + | <li>Repeat characterization of promoter Bba_J23100 using TOP10. </li> |
<li>Repeat characterization of promoter Bba_J23100 using BL21. </li> | <li>Repeat characterization of promoter Bba_J23100 using BL21. </li> | ||
| - | + | </br> | |
| - | + | <li>Second repeat characterization of promoter Bba_J23100 using DH5α. After three times of characterization, the result shown that DM5αis not optimal for this promoter. </li> | |
| - | <li>Second repeat characterization of promoter Bba_J23100 using | + | <li>Second repeat characterization of promoter Bba_J23100 using TOP10. After three times of characterization, the result shown that Top 10 can effectively expresses the protein. </li> |
| - | <li>Second repeat characterization of promoter Bba_J23100 using | + | |
<li>Second repeat characterization of promoter Bba_J23100 using BL21. After three times of characterization, the result shown that BL21 can effectively expresses the protein. </li> | <li>Second repeat characterization of promoter Bba_J23100 using BL21. After three times of characterization, the result shown that BL21 can effectively expresses the protein. </li> | ||
</ol> | </ol> | ||
Latest revision as of 09:06, 26 September 2012
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