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Team:Hong Kong-CUHK/DOC NBK JUL
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<li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | <li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | ||
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<li>Plasmids containing NpSRII-NpHtrII-Ectar -backbone gene were sent to sequencing. </li> | <li>Plasmids containing NpSRII-NpHtrII-Ectar -backbone gene were sent to sequencing. </li> | ||
<li>Make plate with 0.5% soft agar with antibiotics- chloramphenicol. </li> | <li>Make plate with 0.5% soft agar with antibiotics- chloramphenicol. </li> | ||
<li>Make plate with antibiotics –Ampiclin. </li> | <li>Make plate with antibiotics –Ampiclin. </li> | ||
<li>Overlapping PCR of NpSRII-NpHtrII-Ectsr-backbone which the plasmid is responsible in repelling from blue light.</li> | <li>Overlapping PCR of NpSRII-NpHtrII-Ectsr-backbone which the plasmid is responsible in repelling from blue light.</li> | ||
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<li>Direct transformation of NpSRII-NpHtrII-Ectsr-backbone gene, follow by spread plate. </li> | <li>Direct transformation of NpSRII-NpHtrII-Ectsr-backbone gene, follow by spread plate. </li> | ||
<li>Miniprep of picked clones from transformed HsSRI-HsHtrI-Ectsr-backbone cells. </li> | <li>Miniprep of picked clones from transformed HsSRI-HsHtrI-Ectsr-backbone cells. </li> | ||
<li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-backbone gene are incorrect. </li> | <li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-backbone gene are incorrect. </li> | ||
| - | + | </br> | |
<li>Overlapping PCR of NpSRII-NpHtrII-Ectar -backbone which the plasmid is responsible in attracting by blue light.</li> | <li>Overlapping PCR of NpSRII-NpHtrII-Ectar -backbone which the plasmid is responsible in attracting by blue light.</li> | ||
<li>Make LB agar solution</li> | <li>Make LB agar solution</li> | ||
| - | + | </br> | |
<li>Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate. </li> | <li>Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate. </li> | ||
<li>No clone was being found on the plate containing transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | <li>No clone was being found on the plate containing transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | ||
| - | + | </br> | |
<li>Overlapping PCR of NpSRII-NpHtrII-Ectar-backbone which the plasmid is responsible in attracting by blue light.</li> | <li>Overlapping PCR of NpSRII-NpHtrII-Ectar-backbone which the plasmid is responsible in attracting by blue light.</li> | ||
<li>Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate. </li> | <li>Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate. </li> | ||
<li>Pick clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | <li>Pick clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | ||
<li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | <li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. </li> | ||
| - | + | </br> | |
<li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells and was sent for sequencing. </li> | <li>Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectar-backbone cells and was sent for sequencing. </li> | ||
<li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar -backbone gene is correct. </li> | <li>Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar -backbone gene is correct. </li> | ||
<li>The sequenced cells which contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics. </li> | <li>The sequenced cells which contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics. </li> | ||
| - | + | </br> | |
<li>Repeat in testing cells that contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics. </li> | <li>Repeat in testing cells that contain the NpSRII-NpHtrII-Ectar-backbone gene are being cultured in 0.5% soft agar plate for testing using normal light bulb with its corresponding antibiotics. </li> | ||
<li>Pick clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells. </li> | <li>Pick clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells. </li> | ||
Latest revision as of 09:03, 26 September 2012
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