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Team:Macquarie Australia/Protocols/RD
From 2012.igem.org
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| + | <h2>Protocol</h2> | ||
| + | <blockquote><ol><li>Mix 10 µL of the desired DNA/plasmid and 10 µL of the appropriate Master Mix in a PCR tube.</li> | ||
| + | <li>Using a thermocycler, incubate the mixture at 37°C for 30 minutes and then deactivate the enzymes by heating to 80°C for 20 minutes.</li> | ||
| + | <li>Store in a -20°C freezer until DNA required</li></blockquote></ol> | ||
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Revision as of 01:24, 26 September 2012
Restriction Digest
We prepared Master Mixes containing to following volumes,
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Protocol
- Mix 10 µL of the desired DNA/plasmid and 10 µL of the appropriate Master Mix in a PCR tube.
- Using a thermocycler, incubate the mixture at 37°C for 30 minutes and then deactivate the enzymes by heating to 80°C for 20 minutes.
- Store in a -20°C freezer until DNA required
