Team:Macquarie Australia/Protocols/RD

From 2012.igem.org

(Difference between revisions)
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<html>
<html>
<center><h1>Restriction Digest</h1></center>
<center><h1>Restriction Digest</h1></center>
-
<p></p>
+
<p>We prepared Master Mixes containing to following volumes,</p>
 +
<center><table><tr><td><table border="3" cellpadding="4" cellspacing="0">
 +
<tr><td colspan="2">EcoR1 + Spe1 Master Mix</td></tr>
 +
<tr><td>Substrate</td><td>Volume (&micro;L)</td></tr>
 +
<tr><td>10x Buffer</td><td>20</td></tr>
 +
<tr><td>EcoR1</td><td>2.5</td></tr>
 +
<tr><td>Spe1</td><td>2.5</td></tr>
 +
<tr><td>Water</td><td>75</td></tr>
 +
<tr><td>Total</td><td>100</td></tr>
 +
</table></td>
 +
<td><table border="3" cellpadding="4" cellspacing="0">
 +
<tr><td colspan="2">EcoR1 + Spe1 Master Mix</td></tr>
 +
<tr><td>Substrate</td><td>Volume (&micro;L)</td></tr>
 +
<tr><td>10x Buffer</td><td>20</td></tr>
 +
<tr><td>EcoR1</td><td>2.5</td></tr>
 +
<tr><td>Spe1</td><td>2.5</td></tr>
 +
<tr><td>Water</td><td>75</td></tr>
 +
<tr><td>Total</td><td>100</td></tr>
 +
</table></td>
 +
<td><table border="3" cellpadding="4" cellspacing="0">
 +
<tr><td colspan="2">EcoR1 + Spe1 Master Mix</td></tr>
 +
<tr><td>Substrate</td><td>Volume (&micro;L)</td></tr>
 +
<tr><td>10x Buffer</td><td>20</td></tr>
 +
<tr><td>EcoR1</td><td>2.5</td></tr>
 +
<tr><td>Spe1</td><td>2.5</td></tr>
 +
<tr><td>Water</td><td>75</td></tr>
 +
<tr><td>Total</td><td>100</td></tr>
 +
</table></td>
 +
<td><table border="3" cellpadding="4" cellspacing="0">
 +
<tr><td colspan="2">EcoR1 + Spe1 Master Mix</td></tr>
 +
<tr><td>Substrate</td><td>Volume (&micro;L)</td></tr>
 +
<tr><td>10x Buffer</td><td>20</td></tr>
 +
<tr><td>EcoR1</td><td>2.5</td></tr>
 +
<tr><td>Spe1</td><td>2.5</td></tr>
 +
<tr><td>Water</td><td>75</td></tr>
 +
<tr><td>Total</td><td>100</td></tr>
 +
</table></td></table></center>
</html>
</html>

Revision as of 01:19, 26 September 2012



Restriction Digest

We prepared Master Mixes containing to following volumes,

EcoR1 + Spe1 Master Mix
SubstrateVolume (µL)
10x Buffer20
EcoR12.5
Spe12.5
Water75
Total100
EcoR1 + Spe1 Master Mix
SubstrateVolume (µL)
10x Buffer20
EcoR12.5
Spe12.5
Water75
Total100
EcoR1 + Spe1 Master Mix
SubstrateVolume (µL)
10x Buffer20
EcoR12.5
Spe12.5
Water75
Total100
EcoR1 + Spe1 Master Mix
SubstrateVolume (µL)
10x Buffer20
EcoR12.5
Spe12.5
Water75
Total100