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Team:Michigan/Project
From 2012.igem.org
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| - | <p> | + | <p>Recombinases can be used to create responsive genetic elements in biological systems. Further, it is possible to create complex control circuits using combinations of invertible sections. We utilized HbiF to augment an existing recombinase system in <i>Escherichia coli</i> that relied on FimE. A burst of induced, low level expression of one recombinase is expected to invert the promoter flanked by the recombinase binding sites IRR and IRL, triggering strong expression of proteins made downstream. Induced expression of the second recombinase is expected to revert the promoter to its original orientation, triggering a second set of protein expression. The inversion will be sustained across cell divisions with little leaky protein expression and negligible performance degradation after repeated inversions. This system will function as an inheritable memory system with two mutually exclusive states, and can be used as a component of more complex systems. </p> |
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Revision as of 02:59, 26 June 2012
Abstract
Recombinases can be used to create responsive genetic elements in biological systems. Further, it is possible to create complex control circuits using combinations of invertible sections. We utilized HbiF to augment an existing recombinase system in Escherichia coli that relied on FimE. A burst of induced, low level expression of one recombinase is expected to invert the promoter flanked by the recombinase binding sites IRR and IRL, triggering strong expression of proteins made downstream. Induced expression of the second recombinase is expected to revert the promoter to its original orientation, triggering a second set of protein expression. The inversion will be sustained across cell divisions with little leaky protein expression and negligible performance degradation after repeated inversions. This system will function as an inheritable memory system with two mutually exclusive states, and can be used as a component of more complex systems.
HbiF Standardization
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Modeling
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Recombinase Characterization
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