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Team:Bonn/Protocols
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== Lab protocols == | == Lab protocols == | ||
| + | On this page you can find a list of important standard protocols we used in our project. | ||
=== Preparation of chemocompetent DH5-alpha cells === | === Preparation of chemocompetent DH5-alpha cells === | ||
| + | * liquid cultures, OD_600 of 0,6-0,8 | ||
| + | * centrifugation at 4 °C, 4500 g, 10 min | ||
| + | * decantation | ||
| + | * resuspend with 40 ml inoune transformation buffer | ||
| + | * repeat centrifugation, decantation and resuspendation | ||
| + | * centrifuge and decantate again | ||
| + | * resuspend in 20 ml inoune transformation buffer | ||
| + | * add 1,5 ml DMSO | ||
| + | * shock freeze in liquid nitrogen | ||
Revision as of 22:44, 25 September 2012
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Lab protocols
On this page you can find a list of important standard protocols we used in our project.
Preparation of chemocompetent DH5-alpha cells
- liquid cultures, OD_600 of 0,6-0,8
- centrifugation at 4 °C, 4500 g, 10 min
- decantation
- resuspend with 40 ml inoune transformation buffer
- repeat centrifugation, decantation and resuspendation
- centrifuge and decantate again
- resuspend in 20 ml inoune transformation buffer
- add 1,5 ml DMSO
- shock freeze in liquid nitrogen
