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Team:Trieste/data
From 2012.igem.org
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| - | <h3>BBa_K875001, T5 cumate operator:</h3> | + | |
| + | <ul style="list-style-image: url(https://static.igem.org/mediawiki/2012/b/b6/Cuore.jpg);"> | ||
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| + | <li><h3>BBa_K875001, T5 cumate operator:</h3> | ||
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| - | We have dimontrated that it is a very strong and strictly regulated promoter. Thanks to its rapid reactivity it's one of the first candidate for the expression and production of toxin in case of necessity. | + | We have dimontrated that it is a very strong and strictly regulated promoter. Thanks to its rapid reactivity it's one of the first candidate for the expression and production of toxin in case of necessity.</li> |
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| - | <h3>BBa_K875003, CymR:</h3> | + | <li><h3>BBa_K875003, CymR:</h3> |
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| - | We have succesfully expressed the CymR. We have also showed his stringency as repressor of the T5 Cumate Operator that makes the all system a very useful mechanism for an high specificity expression. | + | We have succesfully expressed the CymR. We have also showed his stringency as repressor of the T5 Cumate Operator that makes the all system a very useful mechanism for an high specificity expression. </li> |
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| - | <h3>BBa_K875004, LPP-OmpA-scFv:</h3> | + | <li><h3>BBa_K875004, LPP-OmpA-scFv:</h3> |
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| - | We have verified the production of this protein at high yield. OmpA is a very powerful method for displaying proteins on the external membrane, in this case the antibody scFv 54.6. | + | We have verified the production of this protein at high yield. OmpA is a very powerful method for displaying proteins on the external membrane, in this case the antibody scFv 54.6.</li> |
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Revision as of 22:08, 25 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Data
More
An overview on our system
Our favourite parts:
BBa_K875001, T5 cumate operator:
We have dimontrated that it is a very strong and strictly regulated promoter. Thanks to its rapid reactivity it's one of the first candidate for the expression and production of toxin in case of necessity.BBa_K875003, CymR:
We have succesfully expressed the CymR. We have also showed his stringency as repressor of the T5 Cumate Operator that makes the all system a very useful mechanism for an high specificity expression.BBa_K875004, LPP-OmpA-scFv:
We have verified the production of this protein at high yield. OmpA is a very powerful method for displaying proteins on the external membrane, in this case the antibody scFv 54.6.
The part we have improved:
BBa_K875020, Beta-Glucosidase (Osaka 2010):
