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Team:Trieste/data
From 2012.igem.org
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we have dimontrated that it is a very strong and strictly regulated promoter. Thanks to its rapid reactivity it's one of the first candidate for the expression and production of toxin in case of necessity. | we have dimontrated that it is a very strong and strictly regulated promoter. Thanks to its rapid reactivity it's one of the first candidate for the expression and production of toxin in case of necessity. | ||
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<h3>BBa_K875003, CymR:</h3> | <h3>BBa_K875003, CymR:</h3> | ||
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we have succesfully expressed the CymR. We have also showed his stringency as repressor of the T5 Cumate Operator that makes the all system a very useful mechanism for an high specificity expression. | we have succesfully expressed the CymR. We have also showed his stringency as repressor of the T5 Cumate Operator that makes the all system a very useful mechanism for an high specificity expression. | ||
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<h3>BBa_K875004, LPP-OmpA-scFv:</h3> | <h3>BBa_K875004, LPP-OmpA-scFv:</h3> | ||
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<h2>The part we have improved:</h2> | <h2>The part we have improved:</h2> | ||
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<h3>BBa_K875020, Beta-Glucosidase (Osaka 2010):</h3> | <h3>BBa_K875020, Beta-Glucosidase (Osaka 2010):</h3> | ||
Revision as of 21:55, 25 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Data
More
An overview on our system
Our favourite parts:
BBa_K875001, T5 cumate operator:
we have dimontrated that it is a very strong and strictly regulated promoter. Thanks to its rapid reactivity it's one of the first candidate for the expression and production of toxin in case of necessity.
BBa_K875003, CymR:
we have succesfully expressed the CymR. We have also showed his stringency as repressor of the T5 Cumate Operator that makes the all system a very useful mechanism for an high specificity expression.
BBa_K875004, LPP-OmpA-scFv:
We have verified the production of this protein at high yield. OmpA is a very powerful method for displaying proteins on the external membrane, in this case the antibody scFv 54.6.
The part we have improved:
BBa_K875020, Beta-Glucosidase (Osaka 2010):
