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Team:LMU-Munich/Data/Knockout
From 2012.igem.org
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| - | <font color="#000000"; size="2">'''Plates of germination mutants.''' Plates contained heated (to kill live cells and leave only spores to germinate) cultures and unheated (where colonies may come from germinated spores and the live cells) cultures. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). Mutants used were: <br /> | + | <font color="#000000"; size="2">'''Plates of germination mutants.''' Plates contained heated (to kill live cells and leave only spores to germinate) cultures and unheated (where colonies may come from germinated spores and the live cells) cultures. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). The mistake of photographing empty plates against a background of spots was realized after plates had been discarded. Presence of colonies is noted below, for clarity. Mutants used were: <br /> |
| - | '''WT168''': Wild-type 168 <br /> | + | '''WT168''': Wild-type 168; Heated plate: colonies present; Unheated plate: colonies present <br /> |
| - | '''''spo0A''::tet''': A sporulation-deficient strain <br /> | + | '''''spo0A''::tet''': A sporulation-deficient strain; Heated plate: no colonies; Unheated plate: colonies present <br /> |
| - | '''B42''': ''cwlD''::kan, ''cwlJ''::spec, ''gerD''::cm <br /> | + | '''B42''': ''cwlD''::kan, ''cwlJ''::spec, ''gerD''::cm; Heated plate: colonies present; Unheated plate: colonies present<br /> |
| - | '''B47''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec, ''cwlD''::kan</font> | + | '''B47''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec, ''cwlD''::kan; Heated plate: no colonies; Unheated plate> colonies present</font> |
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Revision as of 21:31, 25 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Knockouts of germination genes
We induced our germination-mutant strains to sporulate in Difco sporulation media. Then we measured the germination rate of mutant spores in a germination assay.
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The plate growth demonstrates the inability of our mutant spores to germinate. We can say that fewer than 1 out of 3x10^7 spores of strains B40, B41, B43, B46, and B47 germinated.
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