"
Team:Paris-Saclay/Project/Notebook/Week 10
From 2012.igem.org
(Difference between revisions)
YohannPetiot (Talk | contribs) |
YohannPetiot (Talk | contribs) |
||
| Line 22: | Line 22: | ||
PCR program used: | PCR program used: | ||
| + | [[File:Week10-3.jpg|330px]] | ||
Revision as of 20:43, 25 September 2012
6th August
- Sending of the B1 sample to sequencing with two pairs of primers
- K-274100 Forward and Reverse
- Plasmid pSB1A2 Forward and Reverse
7th August
- Liquid culture of B1 in order to prepare a glycerol stock
- Receipt of new primers
- 2 Forward
- 3 Reverse
- Plasmid Reverse
8th August
- Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.
PCR program used:
