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Team:Paris-Saclay/Project/Notebook/Week 10
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| - | + | ====6th August==== | |
| - | + | ||
| - | + | *Sending of the B1 sample to sequencing with two pairs of primers | |
| - | + | **K-274100 Forward and Reverse | |
| - | + | **Plasmid pSB1A2 Forward and Reverse | |
| + | |||
| + | |||
| + | ====7th August==== | ||
| + | |||
| + | *Liquid culture of B1 in order to prepare a glycerol stock | ||
| + | **Receipt of new primers | ||
| + | **2 Forward | ||
| + | **3 Reverse | ||
| + | **Plasmid Reverse | ||
| + | |||
| + | |||
| + | ====8th August==== | ||
| + | |||
| + | *Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid. | ||
| + | [[File:Week10-2.jpg|right|300px]] | ||
| + | |||
| + | PCR program used: | ||
Revision as of 20:42, 25 September 2012
6th August
- Sending of the B1 sample to sequencing with two pairs of primers
- K-274100 Forward and Reverse
- Plasmid pSB1A2 Forward and Reverse
7th August
- Liquid culture of B1 in order to prepare a glycerol stock
- Receipt of new primers
- 2 Forward
- 3 Reverse
- Plasmid Reverse
8th August
- Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.
PCR program used:
