(Difference between revisions)

Interactive pater of our construction : Toggle switch option

Put your mouse over a step number/letter to see more

Our parts :

Data for our favorite new parts :

Main Page: Lysostaphin generator for B. subtilis BBa_K802000 : This part associates the Bacillus subtilis Constitutive Promoter (PVeg) with the lysostaphin gene. In our plasmid collection, this part is named pBK23 in the backbone pSB1C3 (cmR) and pBKL28 in the shuttle vector E. coli – B. subtilis. The pBKL28 plasmid was introduced in the strain NM522 to perform tests in E. coli and in the strain 168 to perform tests in Bacillus subtilis.

Main Page: Dispersin generator for B. subtilis Ba_K802001 : This part associates the Bacillus subtilis Constitutive Promoter (PVeg) with the dispersin B gene (DspB). In our plasmid collection, this part is named pBK33 in the backbone Chloramphenicol and pBKH41 in the shuttle vector E. coli – B. subtilis. We worked with the plasmid pBKH41 for the tests and we tried two different genetic backgrounds: the strain NM522 to make test in E. coli and the strain 168 to make test in Bacillus subtilis.

Main Page: Plac(B. subtilis)-RBS(E. coli)-GFP Ba_K802002 :This part is used to determine if the promoter pLac works or not and especially how it works when it is activated by different concentration of the inductor IPTG. Within this part there is pLac from Bacillus subtilis which is the promoter that induces a xylR production to form a positive biofilm. Then a RBS from E coli is added to allow it and finally the GFP which is an easy measurable parameter using the amount of fluorescence.

Main Page: Shuttle vector for E. coli and B. subtilis Ba_K802003:This part is a shuttle vector of 6,5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low plasmid copy number in B. subtilis and a high copy number in E.coli. It has a polylinker containing all 4 of the iGEM sites.

Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802004 : This is a shuttle vector of 6,5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high plasmid copy number in both B. subtilis and E.coli. It has a polylinker containing all 4 of the iGEM sites.

Main Page: iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004 BBa_K802005 : This part is a custom designed polylinker which was cloned into the shuttle vectors BBa_K802004 and BBa_K802003 so that the plasmids would correspond to the iGEM format.