Team:WashU/Week4
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- | The promoter was also re-examined, and it was determined that we should switch to [http://partsregistry.org/Part:BBa_J23119 J23119]. This DNA was rehydrated on the plate and prepared to clone over Friday night. | + | The promoter was also re-examined, and it was determined that we should switch to [http://partsregistry.org/Part:BBa_J23119 J23119], the strongest promoter of the family. We had previously used J23100, a constitutive promoter of the same family, but much weaker than J23119. This DNA was rehydrated on the plate and prepared to clone over Friday night. |
To test whether we had the proper constructs for our different fluorescent proteins, the team ran a gel of the digests, shown below. | To test whether we had the proper constructs for our different fluorescent proteins, the team ran a gel of the digests, shown below. | ||
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<div align = "left"> | <div align = "left"> | ||
==E. coli== | ==E. coli== | ||
- | The transformations from last week seemed to have worked. The CFP is extremely light and looks almost like GFP. | + | The transformations from last week seemed to have worked. The CFP is extremely light and looks almost like GFP. This could be the result of our UV light, which is quite weak. |
- | The team has continued to attempt to biobrick together the necessary genes that create the carotenoid pathway in E. coli (see last week for more information). To do this, the team | + | The team has continued to attempt to biobrick together the necessary genes that create the carotenoid pathway in E. coli (see last week for more information). To do this, the team followed the biobrick assembly protocol once more, synthesizing the upstream, downstream and plasmid parts, and then connected them. In order to increase the efficiency and yield of this assembly, gel purification was used to isolate the plasmid for the biobrick assembly. Below is the gel with the upstream, downstream and plasmid parts. <br> |
https://lh6.googleusercontent.com/-4BRXOgSqUnI/T-SZrvdfUZI/AAAAAAAAAIg/KvIZ0megOSU/s400/ecoli%2520kan.jpg | https://lh6.googleusercontent.com/-4BRXOgSqUnI/T-SZrvdfUZI/AAAAAAAAAIg/KvIZ0megOSU/s400/ecoli%2520kan.jpg | ||
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==Modeling== | ==Modeling== |
Revision as of 21:30, 25 June 2012