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Team:Potsdam Bioware/slider
From 2012.igem.org
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width: 975px; | width: 975px; | ||
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position: relative; | position: relative; | ||
overflow: hidden; | overflow: hidden; | ||
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top:50 px; | top:50 px; | ||
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p.image_description_content{ | p.image_description_content{ | ||
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<img src="https://static.igem.org/mediawiki/2012/8/87/UP12Slide4.jpg"/> | <img src="https://static.igem.org/mediawiki/2012/8/87/UP12Slide4.jpg"/> | ||
<div class="image_description"> | <div class="image_description"> | ||
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Image Description | Image Description | ||
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Revision as of 19:47, 25 September 2012
To produce highly affine antibodies, a CHO (Chinese Hamster Ovary) cell line is transfected with a low affine antibody construct. The plasmid pcDNA5/FRT is used to allow the easy integration of the gene of interest into the CHO cell genome via FLP-In sites. This integration is catalyzed by the FLP-recombinase in the pOG44 plasmid.
