Team:Trieste/parts/2

From 2012.igem.org

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<img src="https://static.igem.org/mediawiki/2012/f/f2/FIG1-OmpA_scFv.png" alt="Omp-scFv"/>
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<img src="https://static.igem.org/mediawiki/2012/4/42/Gel_ompa_scfv_GIMP2.png" alt="Western blo T5LacO-Omp-scFv"/>
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How it works:
How it works:

Revision as of 19:02, 25 September 2012

BBa_K875002

More

Description

This promoter is composed by phage T5 promoter and Lac operator. It is repressed in the presence of the lac repressor protein.

Assembly

Obtained by synthesis.

Results

Trieste Team 2012 used this inducible promotor in the first phase of their project to test every construct that could have been toxic for the host strain, if expressed in a constitutive way.

Western blo T5LacO-Omp-scFv

How it works:
The host strain contains the low copy plasmid pREP4 which constitutively expresses the Lac repressor protein encoded by the lacI gene. The lac repressor binds the operator sequence preventing the transcription. The Lac repressor protein is rapidly inactivated by the binding of isopropyl-β-thiogalactoside (IPTG).

Modelling


Looking forward


Link to the Registry


Università degli studi di Trieste ICGEB Illy Fondazione Cassa di Risparmio
iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012
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