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Team:Bielefeld-Germany/Labjournal/week22
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| - | * '''Team Cultivation & Purification:'''[[File:Bielefeld2012_0924.jpg|300px|thumb|right|'''Figure 1: Cultivation from [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21#Sunday_September_23rd 09/23]''' of ''E. coli'' KRX with <partinfo>BBa_K729006</partinfo> , ''E. coli'' KRX without plasmid (negative control) and with <partinfo>BBa_K863005</partinfo> (positive control). 60 mL in 250 mL flasks without baffles, [https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium], 60 µg/mL chloramphenicol, 37 °C for 12 hours.]] | + | * '''Team Cultivation & Purification:'''[[File:Bielefeld2012_0924.jpg|300px|thumb|right|'''Figure 1: Cultivation from [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21#Sunday_September_23rd 09/23]''' of ''E. coli'' KRX with <partinfo>BBa_K729006</partinfo> , ''E. coli'' KRX without plasmid (negative control) and with <partinfo>BBa_K863005</partinfo> (positive control). 60 mL in 250 mL flasks without baffles,[https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#LB_medium LB-medium] or [https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] for <partinfo>BBa_K863005</partinfo> , 60 µg/mL chloramphenicol, 37 °C for 12 hours.]] |
** For collaboration with [https://2012.igem.org/Team:University_College_London UCL London]: Made celldisruption via sonification and SDS-Page from lysates. The bands were analysed by MALDI-TOF. | ** For collaboration with [https://2012.igem.org/Team:University_College_London UCL London]: Made celldisruption via sonification and SDS-Page from lysates. The bands were analysed by MALDI-TOF. | ||
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Revision as of 18:27, 25 September 2012
Contents |
Week 22 (09/24 - 09/30/12)
Monday September 24th
- Team Cultivation & Purification:
Figure 1: Cultivation from 09/23 of E. coli KRX with <partinfo>BBa_K729006</partinfo> , E. coli KRX without plasmid (negative control) and with <partinfo>BBa_K863005</partinfo> (positive control). 60 mL in 250 mL flasks without baffles,LB-medium or autoinduction medium for <partinfo>BBa_K863005</partinfo> , 60 µg/mL chloramphenicol, 37 °C for 12 hours.
- For collaboration with UCL London: Made celldisruption via sonification and SDS-Page from lysates. The bands were analysed by MALDI-TOF.
Tuesday September 25th
Wednesday September 26th
Thursday September 27th
Friday September 28st
Saturday September 29nd
Sunday September 30rd
Sunday
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