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Team:Trieste/parts/5
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<h2>Description </h2> | <h2>Description </h2> | ||
| + | This construct is designed for the expression of an already descibed engeneered antinorovirus (NoV) monoclonal antibody (mAb 54.6) in fusion with LPP-OmpA. | ||
| + | The antibody is expressed in a small immuno protein (SIP) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker and constant domain (CH3) of heavy chain of human immunoglobulin A (IgA). It has already been reported that the scFv 54.6 (which compones SIP) binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. Alpha isotype CH3 domain homodimerizes confering bivalent binding properties to the antibody. | ||
| + | LPP-OmpA functions as a leader sequence and an anchor to display the scFv ot the bacterial surface.</br></br> | ||
| + | |||
| + | The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, LPP-OmpA, scFv 54.6 antinorovirus, Hystidine tag (6HIS), Terminator (B0015). | ||
| + | |||
</br> | </br> | ||
<h2>Assembly</h2> | <h2>Assembly</h2> | ||
Revision as of 16:14, 25 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
BBa_K875005
More
Description
This construct is designed for the expression of an already descibed engeneered antinorovirus (NoV) monoclonal antibody (mAb 54.6) in fusion with LPP-OmpA. The antibody is expressed in a small immuno protein (SIP) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker and constant domain (CH3) of heavy chain of human immunoglobulin A (IgA). It has already been reported that the scFv 54.6 (which compones SIP) binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. Alpha isotype CH3 domain homodimerizes confering bivalent binding properties to the antibody. LPP-OmpA functions as a leader sequence and an anchor to display the scFv ot the bacterial surface. The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, LPP-OmpA, scFv 54.6 antinorovirus, Hystidine tag (6HIS), Terminator (B0015).Assembly
Results
Modelling
Looking forward
Link to the Registry
